Detailed information of TA system TA186935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1776653..1778161
• Replicon: chromosome
• Accession: NZ_CP068238
• Organism: Pseudomonas aeruginosa strain A39-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JHW37_RS08320
• Protein ID: WP_079383582.1
• Coordinates: 1776653..1777240 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2R3IYR9
• Locus tag: JHW37_RS08325
• Protein ID: WP_043100748.1
• Coordinates: 1777307..1778161 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHW37_RS08315	1772908..1776387	-	3480	WP_053813611.1	hybrid sensor histidine kinase/response regulator	-
JHW37_RS08320	1776653..1777240	+	588	WP_079383582.1	GNAT family N-acetyltransferase	Toxin
JHW37_RS08325	1777307..1778161	+	855	WP_043100748.1	AraC family transcriptional regulator	Antitoxin
JHW37_RS08330	1778257..1780422	+	2166	WP_053816921.1	TonB-dependent receptor family protein	-
JHW37_RS08335	1780597..1782471	+	1875	WP_053816922.1	MFS transporter	-
JHW37_RS08340	1782532..1782741	-	210	WP_003091604.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-147)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21862.19 Da
• Isoelectric Point: 9.8675

>T186935 WP_079383582.1 NZ_CP068238:1776653-1777240 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQGIADQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQFSTAASNLRAQGAIDKLGAQREGLLRN
QRRLAGGRLDDTIVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T186935 NZ_CP089775:682851-683258 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31716.04 Da
• Isoelectric Point: 8.0066

>AT186935 WP_043100748.1 NZ_CP068238:1777307-1778161 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAALGGVELLHARYVEQRFAPHVHSGFALVFIEQGAQRFRHRGGEHLAPWGSMVLINPDEVHTGSK
AHDSGWLYRGYYPEVEQIASVLDELEIRHGGVPRFADSVVHDQPLARAFVHLHRLLADHAGSLETQTCWRETLLALFRRH
ARLPEAPKAGSEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKNGRA
PLDVALELGFADQSHLGRQFKQAYGVAPGEYRAACARSSLTMCE

Nucleotide

• Length: 855 bp

>AT186935 NZ_CP089775:682639-682854 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2R3IYR9