Detailed information of TA system TA186930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3198056..3198687
• Replicon: chromosome
• Accession: NZ_CP068237
• Organism: Klebsiella quasipneumoniae strain YDKL-002

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1Q8YTV3
• Locus tag: JJQ56_RS15525
• Protein ID: WP_012542177.1
• Coordinates: 3198511..3198687 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2X1QFN9
• Locus tag: JJQ56_RS15520
• Protein ID: WP_012542176.1
• Coordinates: 3198056..3198463 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJQ56_RS15480	3194677..3195111	-	435	WP_012542168.1	phage terminase small subunit P27 family	-
JJQ56_RS15485	3195361..3195792	-	432	WP_202023551.1	hypothetical protein	-
JJQ56_RS15490	3195789..3196106	-	318	WP_202023552.1	hypothetical protein	-
JJQ56_RS15495	3196058..3196420	-	363	WP_014228901.1	HNH endonuclease	-
JJQ56_RS15500	3196404..3196565	-	162	WP_202023553.1	hypothetical protein	-
JJQ56_RS15505	3196647..3197123	-	477	WP_172491599.1	DUF2514 domain-containing protein	-
JJQ56_RS15510	3197156..3197686	-	531	WP_023328100.1	lysozyme	-
JJQ56_RS15515	3197664..3197900	-	237	WP_004111739.1	class II holin family protein	-
JJQ56_RS15520	3198056..3198463	-	408	WP_012542176.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JJQ56_RS15525	3198511..3198687	-	177	WP_012542177.1	type II toxin-antitoxin system HicA family toxin	Toxin
JJQ56_RS15530	3199057..3199659	-	603	WP_060657012.1	hypothetical protein	-
JJQ56_RS15535	3199676..3200707	-	1032	WP_046879041.1	DUF968 domain-containing protein	-
JJQ56_RS15540	3200907..3201299	-	393	WP_202023554.1	DNA-binding protein	-
JJQ56_RS15545	3201340..3201579	-	240	WP_050597314.1	hypothetical protein	-
JJQ56_RS15550	3201642..3201875	-	234	WP_202023555.1	DinI-like family protein	-
JJQ56_RS15555	3202137..3202361	-	225	Protein_3053	VapC toxin family PIN domain ribonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3143588..3215318	71730

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6632.83 Da
• Isoelectric Point: 11.0174

>T186930 WP_012542177.1 NZ_CP068237:c3198687-3198511 [Klebsiella quasipneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Nucleotide

• Length: 177 bp

>T186930 NZ_CP089775:367091-367393 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14977.99 Da
• Isoelectric Point: 4.4277

>AT186930 WP_012542176.1 NZ_CP068237:c3198463-3198056 [Klebsiella quasipneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Nucleotide

• Length: 408 bp

>AT186930 NZ_CP089775:366867-367094 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A1Q8YTV3

Antitoxin

• AlphaFold DB: A0A2X1QFN9