Detailed information of TA system TA186918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 36486..37129
• Replicon: plasmid unnamed1
• Accession: NZ_CP068235
• Organism: Leclercia adecarboxylata strain YZ30

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JJN11_RS23660
• Protein ID: WP_159340484.1
• Coordinates: 36486..36902 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: JJN11_RS23665
• Protein ID: WP_159340483.1
• Coordinates: 36899..37129 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJN11_RS23635 (JJN11_23560)	31709..32140	+	432	WP_103181191.1	hypothetical protein	-
JJN11_RS23640	32483..32671	+	189	WP_103181190.1	hypothetical protein	-
JJN11_RS23645 (JJN11_23565)	32934..33887	-	954	Protein_36	integrase core domain-containing protein	-
JJN11_RS23655 (JJN11_23580)	35418..35816	-	399	WP_250366171.1	GAF domain-containing protein	-
JJN11_RS23660 (JJN11_23585)	36486..36902	-	417	WP_159340484.1	type II toxin-antitoxin system VapC family toxin	Toxin
JJN11_RS23665 (JJN11_23590)	36899..37129	-	231	WP_159340483.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JJN11_RS23670 (JJN11_23595)	37463..37723	+	261	WP_159340482.1	type II toxin-antitoxin system ParD family antitoxin	-
JJN11_RS23675 (JJN11_23600)	37710..38006	+	297	WP_159340481.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JJN11_RS23680 (JJN11_23605)	38238..38672	-	435	WP_004388336.1	copper resistance system metallochaperone PcoE	-
JJN11_RS23685 (JJN11_23610)	38888..40288	-	1401	WP_159340480.1	copper resistance membrane spanning protein PcoS	-
JJN11_RS23690 (JJN11_23615)	40285..40965	-	681	WP_001188930.1	copper response regulator transcription factor PcoR	-
JJN11_RS23695 (JJN11_23620)	41020..41949	-	930	WP_000998778.1	copper resistance inner membrane protein PcoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..76263	76263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.40 Da
• Isoelectric Point: 6.4563

>T186918 WP_159340484.1 NZ_CP068235:c36902-36486 [Leclercia adecarboxylata]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRNQRIVISAITYSEIRFGATGPKASPRHTELVDAFCARLDAILPWDSA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLMLEDWAR

Nucleotide

• Length: 417 bp

>T186918 NZ_CP089774:c3066075-3065680 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.80 Da
• Isoelectric Point: 4.3533

>AT186918 WP_159340483.1 NZ_CP068235:c37129-36899 [Leclercia adecarboxylata]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLDKADPDFMAEREDVVTDEGRFDL

Nucleotide

• Length: 231 bp

>AT186918 NZ_CP089774:c3065654-3065388 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA