Detailed information of TA system TA186895

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 1369698..1370340
• Replicon: chromosome
• Accession: NZ_CP068229
• Organism: Shewanella algae strain 150735

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: A0A5N5U1D5
• Locus tag: JKK46_RS06090
• Protein ID: WP_025010549.1
• Coordinates: 1369918..1370340 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: A0A7X5Z2N1
• Locus tag: JKK46_RS06085
• Protein ID: WP_028779782.1
• Coordinates: 1369698..1369937 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKK46_RS06060 (JKK46_06060)	1365381..1366208	+	828	WP_025010545.1	prolipoprotein diacylglyceryl transferase	-
JKK46_RS06065 (JKK46_06065)	1366208..1367002	+	795	WP_025887839.1	thymidylate synthase	-
JKK46_RS06070 (JKK46_06070)	1367119..1368294	+	1176	WP_025010547.1	Na+/H+ antiporter NhaA	-
JKK46_RS06075 (JKK46_06075)	1368291..1368674	+	384	WP_062793820.1	hypothetical protein	-
JKK46_RS06080 (JKK46_06080)	1368691..1369599	+	909	WP_025010548.1	transcriptional activator NhaR	-
JKK46_RS06085 (JKK46_06085)	1369698..1369937	+	240	WP_028779782.1	succinate dehydrogenase assembly factor 2	Antitoxin
JKK46_RS06090 (JKK46_06090)	1369918..1370340	+	423	WP_025010549.1	protein YgfX	Toxin
JKK46_RS06095 (JKK46_06095)	1370358..1371968	-	1611	WP_025010550.1	L-aspartate oxidase	-
JKK46_RS06100 (JKK46_06100)	1372168..1372746	+	579	WP_025010551.1	RNA polymerase sigma factor RpoE	-
JKK46_RS06105 (JKK46_06105)	1372771..1373391	+	621	WP_025887842.1	RseA family anti-sigma factor	-
JKK46_RS06110 (JKK46_06110)	1373401..1374333	+	933	WP_025887844.1	MucB/RseB C-terminal domain-containing protein	-
JKK46_RS06115 (JKK46_06115)	1374344..1374823	+	480	WP_208129192.1	SoxR reducing system RseC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-135)

Antitoxin

• Flavinator of succinate dehydrogenase (5-75)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16309.86 Da
• Isoelectric Point: 7.0359

>T186895 WP_025010549.1 NZ_CP068229:1369918-1370340 [Shewanella algae]
VAGQRHRFQLSSSFSQYLSLTVLAAICLSSFLAWPALDYPFYLLFKYLCLLLMLAGFGLALWRLRCWQLCFWLNELGEGQ
FEPGSSFELSGRPWVSPFVVTFTYQGEDNYGRCWLFADMFDDTDYRHLCRLLLQRSKSAN

Nucleotide

• Length: 423 bp

>T186895 NZ_CP089774:c1578603-1578202 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9247.74 Da
• Isoelectric Point: 5.0633

>AT186895 WP_028779782.1 NZ_CP068229:1369698-1369937 [Shewanella algae]
MSTPRVKWACRRGMLELDVLFQPFVEQHYETLSPEDKALFVRLLECEDPELFAWFMGHEQCPDPQLAQMVVRVRGRAAP

Nucleotide

• Length: 240 bp

>AT186895 NZ_CP089774:c1578857-1578600 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A7X5Z2N1