Detailed information of TA system TA186891

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 1275539..1276181
• Replicon: chromosome
• Accession: NZ_CP068228
• Organism: Shewanella algae strain G1

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: -
• Locus tag: JKK44_RS05855
• Protein ID: WP_146507781.1
• Coordinates: 1275759..1276181 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: A0A7X5Z2N1
• Locus tag: JKK44_RS05850
• Protein ID: WP_028779782.1
• Coordinates: 1275539..1275778 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKK44_RS05825 (JKK44_05825)	1271222..1272049	+	828	WP_071238279.1	prolipoprotein diacylglyceryl transferase	-
JKK44_RS05830 (JKK44_05830)	1272049..1272843	+	795	WP_071238278.1	thymidylate synthase	-
JKK44_RS05835 (JKK44_05835)	1272960..1274135	+	1176	WP_025010547.1	Na+/H+ antiporter NhaA	-
JKK44_RS05840 (JKK44_05840)	1274132..1274515	+	384	WP_243757077.1	hypothetical protein	-
JKK44_RS05845 (JKK44_05845)	1274532..1275440	+	909	WP_025010548.1	transcriptional activator NhaR	-
JKK44_RS05850 (JKK44_05850)	1275539..1275778	+	240	WP_028779782.1	succinate dehydrogenase assembly factor 2	Antitoxin
JKK44_RS05855 (JKK44_05855)	1275759..1276181	+	423	WP_146507781.1	protein YgfX	Toxin
JKK44_RS05860 (JKK44_05860)	1276199..1277809	-	1611	WP_025010550.1	L-aspartate oxidase	-
JKK44_RS05865 (JKK44_05865)	1278009..1278587	+	579	WP_025010551.1	RNA polymerase sigma factor RpoE	-
JKK44_RS05870 (JKK44_05870)	1278612..1279232	+	621	WP_107110666.1	RseA family anti-sigma factor	-
JKK44_RS05875 (JKK44_05875)	1279242..1280174	+	933	WP_025010553.1	MucB/RseB C-terminal domain-containing protein	-
JKK44_RS05880 (JKK44_05880)	1280185..1280664	+	480	WP_144132571.1	SoxR reducing system RseC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-135)

Antitoxin

• Flavinator of succinate dehydrogenase (5-75)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16280.90 Da
• Isoelectric Point: 7.9361

>T186891 WP_146507781.1 NZ_CP068228:1275759-1276181 [Shewanella algae]
VAGQRHRFQLSSSFSQYLSLTVLAAICLSSFLAWPALDYPFYLLFKYLCLLLMLAGFGLALWRLRCWQLCFWLNELGEGQ
FEPGSSFKLSGKPWVSPFVVTFTYQGEDNYGRCWLFADMFDDTDYRHLCRLLLQRSKSAN

Nucleotide

• Length: 423 bp

>T186891 NZ_CP089774:1246472-1246846 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9247.74 Da
• Isoelectric Point: 5.0633

>AT186891 WP_028779782.1 NZ_CP068228:1275539-1275778 [Shewanella algae]
MSTPRVKWACRRGMLELDVLFQPFVEQHYETLSPEDKALFVRLLECEDPELFAWFMGHEQCPDPQLAQMVVRVRGRAAP

Nucleotide

• Length: 240 bp

>AT186891 NZ_CP089774:1246278-1246475 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X5Z2N1