Detailed information of TA system TA186887

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 3581686..3582328
• Replicon: chromosome
• Accession: NZ_CP068226
• Organism: Shewanella algae strain A291

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: A0A5N5U1D5
• Locus tag: JKK45_RS15730
• Protein ID: WP_025010549.1
• Coordinates: 3581686..3582108 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: A0A7X5Z2N1
• Locus tag: JKK45_RS15735
• Protein ID: WP_028779782.1
• Coordinates: 3582089..3582328 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKK45_RS15705 (JKK45_15710)	3577203..3577682	-	480	WP_025010554.1	SoxR reducing system RseC family protein	-
JKK45_RS15710 (JKK45_15715)	3577693..3578625	-	933	WP_025887844.1	MucB/RseB C-terminal domain-containing protein	-
JKK45_RS15715 (JKK45_15720)	3578635..3579255	-	621	WP_208123636.1	RseA family anti-sigma factor	-
JKK45_RS15720 (JKK45_15725)	3579280..3579858	-	579	WP_025010551.1	RNA polymerase sigma factor RpoE	-
JKK45_RS15725 (JKK45_15730)	3580058..3581668	+	1611	WP_025010550.1	L-aspartate oxidase	-
JKK45_RS15730 (JKK45_15735)	3581686..3582108	-	423	WP_025010549.1	protein YgfX	Toxin
JKK45_RS15735 (JKK45_15740)	3582089..3582328	-	240	WP_028779782.1	succinate dehydrogenase assembly factor 2	Antitoxin
JKK45_RS15740 (JKK45_15745)	3582427..3583335	-	909	WP_025010548.1	transcriptional activator NhaR	-
JKK45_RS15745 (JKK45_15750)	3583352..3583735	-	384	WP_062793820.1	hypothetical protein	-
JKK45_RS15750 (JKK45_15755)	3583732..3584907	-	1176	WP_025010547.1	Na+/H+ antiporter NhaA	-
JKK45_RS15755 (JKK45_15760)	3585024..3585818	-	795	WP_025887839.1	thymidylate synthase	-
JKK45_RS15760 (JKK45_15765)	3585818..3586645	-	828	WP_025010545.1	prolipoprotein diacylglyceryl transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-135)

Antitoxin

• Flavinator of succinate dehydrogenase (5-75)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16309.86 Da
• Isoelectric Point: 7.0359

>T186887 WP_025010549.1 NZ_CP068226:c3582108-3581686 [Shewanella algae]
VAGQRHRFQLSSSFSQYLSLTVLAAICLSSFLAWPALDYPFYLLFKYLCLLLMLAGFGLALWRLRCWQLCFWLNELGEGQ
FEPGSSFELSGRPWVSPFVVTFTYQGEDNYGRCWLFADMFDDTDYRHLCRLLLQRSKSAN

Nucleotide

• Length: 423 bp

>T186887 NZ_CP089774:846618-847046 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9247.74 Da
• Isoelectric Point: 5.0633

>AT186887 WP_028779782.1 NZ_CP068226:c3582328-3582089 [Shewanella algae]
MSTPRVKWACRRGMLELDVLFQPFVEQHYETLSPEDKALFVRLLECEDPELFAWFMGHEQCPDPQLAQMVVRVRGRAAP

Nucleotide

• Length: 240 bp

>AT186887 NZ_CP089774:846337-846594 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7X5Z2N1