Detailed information of TA system TA186873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4291688..4292304
• Replicon: chromosome
• Accession: NZ_CP068215
• Organism: Enterobacter hormaechei strain FDAARGOS_1078

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6I35_RS20650
• Protein ID: WP_077266491.1
• Coordinates: 4291688..4292059 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: I6I35_RS20655
• Protein ID: WP_015569912.1
• Coordinates: 4292062..4292304 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I35_RS20635	4289188..4290090	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
I6I35_RS20640	4290087..4290722	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
I6I35_RS20645	4290719..4291648	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
I6I35_RS20650	4291688..4292059	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6I35_RS20655	4292062..4292304	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6I35_RS20660	4292503..4293423	+	921	WP_032652004.1	alpha/beta hydrolase	-
I6I35_RS20665	4293432..4294373	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
I6I35_RS20670	4294418..4294855	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6I35_RS20675	4294852..4295733	-	882	WP_003861949.1	virulence factor BrkB family protein	-
I6I35_RS20680	4295727..4296326	-	600	WP_003861947.1	glucose-1-phosphatase	-
I6I35_RS20685	4296445..4297245	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T186873 WP_077266491.1 NZ_CP068215:c4292059-4291688 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T186873 NZ_CP089774:367660-368085 [Mycobacterium tuberculosis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT186873 WP_015569912.1 NZ_CP068215:c4292304-4292062 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT186873 NZ_CP089774:367442-367663 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3