Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1158142..1158939 Replicon chromosome
Accession NZ_CP068215
Organism Enterobacter hormaechei strain FDAARGOS_1078

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag I6I35_RS05545 Protein ID WP_032647926.1
Coordinates 1158418..1158939 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag I6I35_RS05540 Protein ID WP_001303459.1
Coordinates 1158142..1158411 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I35_RS05510 1153856..1154737 + 882 WP_032653182.1 hypothetical protein -
I6I35_RS05515 1154734..1155165 + 432 WP_050597034.1 hypothetical protein -
I6I35_RS05520 1155643..1155870 - 228 WP_006809324.1 YccJ family protein -
I6I35_RS05525 1155889..1156485 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
I6I35_RS05530 1156876..1157046 + 171 WP_001273664.1 general stress protein -
I6I35_RS05535 1157163..1158068 + 906 WP_032653183.1 DMT family transporter -
I6I35_RS05540 1158142..1158411 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
I6I35_RS05545 1158418..1158939 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
I6I35_RS05550 1158998..1160320 - 1323 WP_032653185.1 pyrimidine utilization transport protein G -
I6I35_RS05555 1160342..1160833 - 492 WP_032653187.1 pyrimidine utilization flavin reductase protein F -
I6I35_RS05560 1160846..1161436 - 591 WP_017693662.1 malonic semialdehyde reductase -
I6I35_RS05565 1161446..1162246 - 801 WP_023296287.1 pyrimidine utilization protein D -
I6I35_RS05570 1162254..1162640 - 387 WP_015570870.1 pyrimidine utilization protein C -
I6I35_RS05575 1162652..1163341 - 690 WP_032653188.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T186859 WP_032647926.1 NZ_CP068215:1158418-1158939 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T186859 NZ_CP089773:c3072857-3072462 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT186859 WP_001303459.1 NZ_CP068215:1158142-1158411 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT186859 NZ_CP089773:c3072436-3072170 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References