Detailed information of TA system TA186827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 48880..49464
• Replicon: plasmid unnamed1
• Accession: NZ_CP068202
• Organism: Acinetobacter johnsonii strain FDAARGOS_1092

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: I6I49_RS00275
• Protein ID: WP_000286964.1
• Coordinates: 48880..49182 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: I6I49_RS00280
• Protein ID: WP_000985610.1
• Coordinates: 49183..49464 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I49_RS00250	45176..45472	+	297	Protein_49	sensor domain-containing diguanylate cyclase	-
I6I49_RS00255	45504..47129	-	1626	WP_201949790.1	IS66 family transposase	-
I6I49_RS00260	47204..47539	-	336	WP_004642106.1	IS66 family insertion sequence element accessory protein TnpB	-
I6I49_RS00265	47536..47919	-	384	WP_043974824.1	IS66 family insertion sequence hypothetical protein	-
I6I49_RS00270	48008..48664	+	657	Protein_53	GGDEF domain-containing protein	-
I6I49_RS00275	48880..49182	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I49_RS00280	49183..49464	+	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
I6I49_RS00285	49551..50093	-	543	WP_058951821.1	cysteine hydrolase	-
I6I49_RS00290	50135..50620	-	486	Protein_57	IS3 family transposase	-
I6I49_RS00295	50700..51940	-	1241	Protein_58	group II intron reverse transcriptase/maturase	-
I6I49_RS00300	52454..53202	-	749	Protein_59	IS3 family transposase	-
I6I49_RS00305	53423..53848	+	426	WP_201949792.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..183617	183617
	-	inside	IScluster/Tn	-	-	45504..55080	9576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T186827 WP_000286964.1 NZ_CP068202:48880-49182 [Acinetobacter johnsonii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T186827 NZ_CP089773:c760826-760389 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT186827 WP_000985610.1 NZ_CP068202:49183-49464 [Acinetobacter johnsonii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT186827 NZ_CP089773:c761077-760823 [Mycobacterium tuberculosis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94