Detailed information of TA system TA186826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 26370..26898
• Replicon: plasmid unnamed1
• Accession: NZ_CP068202
• Organism: Acinetobacter johnsonii strain FDAARGOS_1092

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: I6I49_RS00150
• Protein ID: WP_119686978.1
• Coordinates: 26370..26663 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9B5Q7
• Locus tag: I6I49_RS00155
• Protein ID: WP_000246756.1
• Coordinates: 26653..26898 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I49_RS00125	22080..22447	-	368	Protein_24	transposase	-
I6I49_RS00130	22745..23086	+	342	WP_201949780.1	hypothetical protein	-
I6I49_RS00135	23173..24305	+	1133	Protein_26	IS3 family transposase	-
I6I49_RS00140	24369..24785	+	417	WP_201949782.1	hypothetical protein	-
I6I49_RS00145	24953..26287	+	1335	WP_201949784.1	ISNCY family transposase	-
I6I49_RS00150	26370..26663	-	294	WP_119686978.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I49_RS00155	26653..26898	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
I6I49_RS00160	27013..28347	-	1335	WP_201949784.1	ISNCY family transposase	-
I6I49_RS00165	28693..29076	+	384	WP_168386657.1	transposase	-
I6I49_RS00170	29073..29408	+	336	WP_168386658.1	IS66 family insertion sequence element accessory protein TnpB	-
I6I49_RS00175	29483..30862	+	1380	Protein_34	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..183617	183617
	-	inside	IScluster/Tn	-	-	4469..40149	35680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11319.59 Da
• Isoelectric Point: 10.5382

>T186826 WP_119686978.1 NZ_CP068202:c26663-26370 [Acinetobacter johnsonii]
MTYKLLRHKDFKAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T186826 NZ_CP089773:c760826-760389 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9235.61 Da
• Isoelectric Point: 4.3142

>AT186826 WP_000246756.1 NZ_CP068202:c26898-26653 [Acinetobacter johnsonii]
MATVNFRVDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT186826 NZ_CP089773:c760279-760034 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Antitoxin

• AlphaFold DB: N9B5Q7