Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 53535..54157 Replicon plasmid unnamed3
Accession NZ_CP068198
Organism Acinetobacter johnsonii strain FDAARGOS_1093

Toxin (Protein)


Gene name vapC Uniprot ID A0A7H8SKU4
Locus tag I6I50_RS18035 Protein ID WP_063454755.1
Coordinates 53762..54157 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R9B9I8
Locus tag I6I50_RS18030 Protein ID WP_000401521.1
Coordinates 53535..53765 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I50_RS18000 49231..49566 + 336 WP_035363642.1 hypothetical protein -
I6I50_RS18005 49616..49795 + 180 WP_016658770.1 hypothetical protein -
I6I50_RS18010 49792..50178 + 387 WP_004769969.1 type II toxin-antitoxin system death-on-curing family toxin -
I6I50_RS18015 50180..50731 - 552 WP_016658769.1 macro domain-containing protein -
I6I50_RS18020 50737..51420 - 684 WP_016658768.1 hypothetical protein -
I6I50_RS18025 51630..52955 + 1326 Protein_52 ISNCY family transposase -
I6I50_RS18030 53535..53765 + 231 WP_000401521.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I6I50_RS18035 53762..54157 + 396 WP_063454755.1 type II toxin-antitoxin system VapC family toxin Toxin
I6I50_RS18040 54318..54749 + 432 WP_063454756.1 hypothetical protein -
I6I50_RS18045 54871..55470 - 600 Protein_56 ISNCY family transposase -
I6I50_RS18050 55533..56665 - 1133 Protein_57 IS3 family transposase -
I6I50_RS18055 56752..57351 - 600 Protein_58 helix-turn-helix domain-containing protein -
I6I50_RS18060 57559..58356 + 798 Protein_59 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..206659 206659
- inside IScluster/Tn - - 28170..69649 41479


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-124)

Antitoxin

SpoVT-AbrB domain

(7-37)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14897.23 Da        Isoelectric Point: 7.1633

>T186825 WP_063454755.1 NZ_CP068198:53762-54157 [Acinetobacter johnsonii]
MIYLLDTNICIYVINNKPQQVFERFKQYQLGQLAISSITASELAFGVEKSGSERNKKALNKFLSPLEILPYDEQAIWHYA
KLRQNLQSTGKPIGSLDMLIAAHALALDVVLVTNNTKEFERIDGLKLENWV

Download         Length: 396 bp

>T186825 NZ_CP089773:c760047-759739 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8759.04 Da        Isoelectric Point: 4.7708

>AT186825 WP_000401521.1 NZ_CP068198:53535-53765 [Acinetobacter johnsonii]
MEIAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIDNPWDVMLEAINEFEVGFQLERADQGEQLREEFR

Download         Length: 231 bp

>AT186825 NZ_CP089773:c760279-760034 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H8SKU4

Antitoxin

Source ID Structure
AlphaFold DB A0A2N6V6E6

References