Detailed information of TA system TA186824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1005..1593
• Replicon: plasmid unnamed2
• Accession: NZ_CP068197
• Organism: Acinetobacter johnsonii strain FDAARGOS_1093

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: I6I50_RS17720
• Protein ID: WP_201707226.1
• Coordinates: 1306..1593 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I6I50_RS17715
• Protein ID: WP_201707225.1
• Coordinates: 1005..1319 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I50_RS17705	16..621	+	606	WP_201707223.1	helix-turn-helix domain-containing protein	-
I6I50_RS17710	614..913	+	300	WP_201707224.1	hypothetical protein	-
I6I50_RS17715	1005..1319	-	315	WP_201707225.1	BrnA antitoxin family protein	Antitoxin
I6I50_RS17720	1306..1593	-	288	WP_201707226.1	BrnT family toxin	Toxin
I6I50_RS17725	2104..3591	+	1488	WP_005146104.1	SulP family inorganic anion transporter	-
I6I50_RS17730	3604..4455	+	852	WP_002115246.1	universal stress protein	-
I6I50_RS17735	4687..5241	-	555	WP_201707227.1	plasmid replication DNA-binding protein	-
I6I50_RS17740	5310..6233	-	924	WP_005198814.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10467	10467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11263.65 Da
• Isoelectric Point: 5.2134

>T186824 WP_201707226.1 NZ_CP068197:c1593-1306 [Acinetobacter johnsonii]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQITKAERDKYEHG

Nucleotide

• Length: 288 bp

>T186824 NZ_CP089773:c758421-758038 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGACGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12204.75 Da
• Isoelectric Point: 6.7282

>AT186824 WP_201707225.1 NZ_CP068197:c1319-1005 [Acinetobacter johnsonii]
MSTVRYSRKELNEKFSDKQDAEIEHLLAKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT186824 NZ_CP089773:c758671-758516 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG