Detailed information of TA system TA186823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 882681..883269
• Replicon: chromosome
• Accession: NZ_CP068195
• Organism: Acinetobacter johnsonii strain FDAARGOS_1093

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A2W5RB36
• Locus tag: I6I50_RS04470
• Protein ID: WP_039890600.1
• Coordinates: 882982..883269 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I6I50_RS04465
• Protein ID: WP_126034950.1
• Coordinates: 882681..882995 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I50_RS04430	877787..878080	+	294	WP_119686978.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6I50_RS04435	878472..879544	+	1073	Protein_849	IS3 family transposase	-
I6I50_RS04440	879572..879910	-	339	WP_178896424.1	transposase	-
I6I50_RS04445	880002..880406	-	405	WP_004730644.1	transposase family protein	-
I6I50_RS04450	880628..881542	+	915	WP_201707174.1	hypothetical protein	-
I6I50_RS04455	881539..882003	+	465	WP_126034954.1	hypothetical protein	-
I6I50_RS04460	881990..882616	+	627	WP_126034952.1	hypothetical protein	-
I6I50_RS04465	882681..882995	-	315	WP_126034950.1	BrnA antitoxin family protein	Antitoxin
I6I50_RS04470	882982..883269	-	288	WP_039890600.1	BrnT family toxin	Toxin
I6I50_RS04475	883531..884865	-	1335	WP_010326716.1	ISNCY family transposase	-
I6I50_RS04480	885481..886605	+	1125	WP_126034948.1	ABC transporter ATP-binding protein	-
I6I50_RS04485	886627..887643	+	1017	WP_005401415.1	extracellular solute-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	katA	851950..895504	43554
	-	inside	IScluster/Tn	-	katA	872702..884865	12163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11317.74 Da
• Isoelectric Point: 6.0889

>T186823 WP_039890600.1 NZ_CP068195:c883269-882982 [Acinetobacter johnsonii]
MEQYFEWDEAKNRKKQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T186823 NZ_CP089773:722803-723210 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12233.90 Da
• Isoelectric Point: 10.0927

>AT186823 WP_126034950.1 NZ_CP068195:c882995-882681 [Acinetobacter johnsonii]
MSMVRYTRKELNENFSDKQDAEIKRLLAKGTVPDDQLDLSDIPEITDWSNAVRHGQFYRPVKQQTSVRLDADVLAWLKSQ
GKGYQTRMNKILREAMLNERKNHR

Nucleotide

• Length: 315 bp

>AT186823 NZ_CP089773:722546-722806 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A2W5RB36