Detailed information of TA system TA186819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 241..829
• Replicon: plasmid unnamed6
• Accession: NZ_CP068191
• Organism: Acinetobacter johnsonii strain FDAARGOS_1094

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8QJ17
• Locus tag: I6I51_RS17265
• Protein ID: WP_004650370.1
• Coordinates: 542..829 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: I6I51_RS17260
• Protein ID: WP_201704359.1
• Coordinates: 241..555 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I51_RS17260	241..555	-	315	WP_201704359.1	BrnA antitoxin family protein	Antitoxin
I6I51_RS17265	542..829	-	288	WP_004650370.1	BrnT family toxin	Toxin
I6I51_RS17270	1323..1643	-	321	WP_005404017.1	hypothetical protein	-
I6I51_RS17275	2104..2640	-	537	WP_201704369.1	plasmid replication DNA-binding protein	-
I6I51_RS17280	2707..3636	-	930	WP_017401951.1	replication initiation protein RepM	-
I6I51_RS17285	4847..5740	+	894	WP_201704360.1	integrase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..14375	14375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11165.56 Da
• Isoelectric Point: 4.7494

>T186819 WP_004650370.1 NZ_CP068191:c829-542 [Acinetobacter johnsonii]
MELYFEWDEAKNQKNQKKHDVSFETASLIFDDPLRISIQDRHTGGEERWQTIGMVKGVLMLLVAHTIFDEDDCEIIRIIS
AREVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T186819 NZ_CP089773:c702208-701795 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12216.86 Da
• Isoelectric Point: 8.4628

>AT186819 WP_201704359.1 NZ_CP068191:c555-241 [Acinetobacter johnsonii]
MSMVRYTRKELNENFSDKQDAEIKRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT186819 NZ_CP089773:c702441-702205 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: N8QJ17