Detailed information of TA system TA186804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 6678..7678
• Replicon: plasmid unnamed
• Accession: NZ_CP068163
• Organism: Corynebacterium glucuronolyticum strain FDAARGOS_1111

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: I6I68_RS12960
• Protein ID: WP_086892511.1
• Coordinates: 6678..7232 (-)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: I6I68_RS12965
• Protein ID: WP_086892529.1
• Coordinates: 7229..7678 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I68_RS12940	4726..4962	-	237	WP_201829662.1	peptide transporter	-
I6I68_RS12945	4973..5569	-	597	WP_201829660.1	ParA family protein	-
I6I68_RS12950	5957..6154	-	198	WP_201829658.1	CPBP family intramembrane metalloprotease	-
I6I68_RS12955	6241..6540	+	300	WP_201842009.1	transposase	-
I6I68_RS12960	6678..7232	-	555	WP_086892511.1	PIN domain-containing protein	Toxin
I6I68_RS12965	7229..7678	-	450	WP_086892529.1	helix-turn-helix domain-containing protein	Antitoxin
I6I68_RS12970	7860..8117	+	258	WP_201842011.1	hypothetical protein	-
I6I68_RS12975	8117..8710	+	594	WP_201829654.1	recombinase family protein	-
I6I68_RS12980	9035..9184	+	150	WP_201829652.1	hypothetical protein	-
I6I68_RS12985	9938..10126	+	189	WP_070769503.1	integrase core domain-containing protein	-
I6I68_RS12990	10163..10411	-	249	Protein_11	transposase	-
I6I68_RS12995	11031..11264	+	234	WP_201829650.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..20681	20681
	-	flank	IS/Tn	-	-	10115..10411	296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Helix-turn-helix domain, group 17 (79-126)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20528.32 Da
• Isoelectric Point: 4.3345

>T186804 WP_086892511.1 NZ_CP068163:c7232-6678 [Corynebacterium glucuronolyticum]
VSGFTVIYDANVLYPNMLRDILIRLAGTGIYQARWTEDILDEVFRNLQANRPDLDPSKLRRTRELMCRAVDDCLVTGYED
LIESLSLPDPDDRHVLAAAIRCGAQVIVTENARDFPQDVMDKYGIEIQGADEFLCDQIDLFGTRVHQAITYAAAAFKNPP
MTIDDVLDALARAGAPSAADLLRR

Nucleotide

• Length: 555 bp

>T186804 NZ_CP089772:c3138253-3137861 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16490.76 Da
• Isoelectric Point: 6.0987

>AT186804 WP_086892529.1 NZ_CP068163:c7678-7229 [Corynebacterium glucuronolyticum]
MAYLQDNEIASTAATAREALQKINRVLSDTSTNKDVKVVVEQSDEVIRLPREVATVLREILVNSVAGRSVSVIPMRAELT
TQQAANLLNVSRPHVIKLMDDGILPGHKVGTHRRIYAADVQAYKHQRDIEARTAADDLTALTEEMGLYE

Nucleotide

• Length: 450 bp

>AT186804 NZ_CP089772:c3138465-3138250 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA