Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4773562..4774200 Replicon chromosome
Accession NZ_CP068155
Organism Escherichia coli strain FDAARGOS_1077

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag I6I34_RS24315 Protein ID WP_000813794.1
Coordinates 4774024..4774200 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag I6I34_RS24310 Protein ID WP_001270286.1
Coordinates 4773562..4773978 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I34_RS24290 4768714..4769655 - 942 WP_001251313.1 ABC transporter permease -
I6I34_RS24295 4769656..4770669 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
I6I34_RS24300 4770687..4771832 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
I6I34_RS24305 4772077..4773483 - 1407 WP_000760586.1 PLP-dependent aminotransferase family protein -
I6I34_RS24310 4773562..4773978 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
I6I34_RS24315 4774024..4774200 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
I6I34_RS24320 4774422..4774652 + 231 WP_000494244.1 YncJ family protein -
I6I34_RS24325 4774744..4776705 - 1962 WP_001355676.1 23S rRNA 5-hydroxycytidine C2501 synthase -
I6I34_RS24330 4776778..4777314 - 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
I6I34_RS24335 4777367..4778581 + 1215 WP_071597387.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4778621..4779886 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T186797 WP_000813794.1 NZ_CP068155:c4774200-4774024 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T186797 NZ_CP089772:c2871526-2871131 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT186797 WP_001270286.1 NZ_CP068155:c4773978-4773562 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT186797 NZ_CP089772:c2871768-2871523 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References