Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1743439..1743936 Replicon chromosome
Accession NZ_CP068155
Organism Escherichia coli strain FDAARGOS_1077

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6I34_RS09670 Protein ID WP_001173343.1
Coordinates 1743439..1743612 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag I6I34_RS09675 Protein ID WP_071597404.1
Coordinates 1743640..1743936 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I34_RS09660 1740104..1741561 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
I6I34_RS09665 1741798..1743315 + 1518 WP_001295074.1 lysine--tRNA ligase -
I6I34_RS09670 1743439..1743612 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6I34_RS09675 1743640..1743936 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6I34_RS09680 1744163..1744435 - 273 WP_000405647.1 N-acetyltransferase -
I6I34_RS09685 1744447..1744677 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
I6I34_RS09690 1744858..1746489 + 1632 WP_001216490.1 sensor histidine kinase -
I6I34_RS09695 1746486..1747205 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T186780 WP_001173343.1 NZ_CP068155:c1743612-1743439 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T186780 NZ_CP068155:c1743612-1743439 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT186780 WP_071597404.1 NZ_CP068155:c1743936-1743640 [Escherichia coli]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT186780 NZ_CP068155:c1743936-1743640 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References