Detailed information of TA system TA186764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5709104..5709744
• Replicon: chromosome
• Accession: NZ_CP068151
• Organism: Pseudomonas fluorescens strain FDAARGOS_1088

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A0W0MYI4
• Locus tag: I6I45_RS26030
• Protein ID: WP_014719602.1
• Coordinates: 5709331..5709744 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: I6I45_RS26025
• Protein ID: WP_103452516.1
• Coordinates: 5709104..5709334 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I45_RS26005	5705362..5706321	+	960	WP_014719606.1	LysR family transcriptional regulator	-
I6I45_RS26010	5706413..5707060	-	648	WP_020303103.1	HAD-IB family hydrolase	-
I6I45_RS26015	5707133..5707306	-	174	Protein_5120	HNH endonuclease	-
I6I45_RS26020	5707373..5708623	-	1251	WP_014719604.1	ribonucleotide-diphosphate reductase subunit beta	-
I6I45_RS26025	5709104..5709334	+	231	WP_103452516.1	AbrB family transcriptional regulator	Antitoxin
I6I45_RS26030	5709331..5709744	+	414	WP_014719602.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6I45_RS26035	5709882..5710151	-	270	WP_014719601.1	DUF2790 domain-containing protein	-
I6I45_RS26040	5710550..5712505	+	1956	WP_127882265.1	acetate--CoA ligase	-
I6I45_RS26045	5713202..5713987	+	786	WP_020302951.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15188.53 Da
• Isoelectric Point: 7.6998

>T186764 WP_014719602.1 NZ_CP068151:5709331-5709744 [Pseudomonas fluorescens]
VTTYMLDTCICSFIMRERPECVLKRLALEVARSNRIVISAITYAEMRYGQIGKKASSKHKTLVDEFVKRLDEVLPWGVAA
VDATVEVKRSLTEQGAIIGQNDTAIAGHAIASGCTLVTNNIREFSRVAGLSYEDWVH

Nucleotide

• Length: 414 bp

>T186764 NZ_CP089772:718051-718446 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8653.71 Da
• Isoelectric Point: 5.0651

>AT186764 WP_103452516.1 NZ_CP068151:5709104-5709334 [Pseudomonas fluorescens]
MRTVSIFKNGKNQAIRFPNDMAYEGVGELEITRNGDTITLRPVRPSWGSLSELPRADEDFLQKRQDVVGDEGRFNL

Nucleotide

• Length: 231 bp

>AT186764 NZ_CP089772:717797-718051 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0W0MYI4