Detailed information of TA system TA186762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 3317962..3318509
• Replicon: chromosome
• Accession: NZ_CP068151
• Organism: Pseudomonas fluorescens strain FDAARGOS_1088

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S6AKK9
• Locus tag: I6I45_RS15095
• Protein ID: WP_016502317.1
• Coordinates: 3318234..3318509 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6AY38
• Locus tag: I6I45_RS15090
• Protein ID: WP_016502318.1
• Coordinates: 3317962..3318234 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I45_RS15060	3313334..3313765	+	432	WP_003425050.1	Hg(II)-responsive transcriptional regulator	-
I6I45_RS15065	3314055..3314342	+	288	Protein_2975	transposase	-
I6I45_RS15070	3314339..3315808	+	1470	WP_034139926.1	ATP-binding protein	-
I6I45_RS15075	3315994..3316557	-	564	WP_019334898.1	recombinase family protein	-
I6I45_RS15080	3316788..3317528	+	741	WP_019334897.1	DUF2807 domain-containing protein	-
I6I45_RS15085	3317535..3317948	+	414	WP_033901201.1	hypothetical protein	-
I6I45_RS15090	3317962..3318234	+	273	WP_016502318.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6I45_RS15095	3318234..3318509	+	276	WP_016502317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I45_RS15100	3318784..3320055	+	1272	WP_023082049.1	IS4-like element ISPa1635 family transposase	-
I6I45_RS15105	3320431..3320826	+	396	WP_080034397.1	GtrA family protein	-
I6I45_RS15110	3320807..3321793	+	987	WP_100226690.1	glycosyltransferase family 2 protein	-
I6I45_RS15115	3321793..3323208	+	1416	WP_019334892.1	glycosyltransferase family 39 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3315994..3327154	11160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10702.22 Da
• Isoelectric Point: 7.2020

>T186762 WP_016502317.1 NZ_CP068151:3318234-3318509 [Pseudomonas fluorescens]
MELKWTSKALSDIARLYEFLASVNQPAAARTVQQLTAAPSTLLANPRIGERLEEFDPRDVRRIQIGHYEMRYEIVDSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T186762 NZ_CP089772:704873-705274 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10074.43 Da
• Isoelectric Point: 5.7066

>AT186762 WP_016502318.1 NZ_CP068151:3317962-3318234 [Pseudomonas fluorescens]
MATTAKTRSVTAHVPVQLAEKVDLMAERLERSKNWIVKQALAAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWAESL
STDTPLPVPR

Nucleotide

• Length: 273 bp

>AT186762 NZ_CP089772:704631-704876 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: S6AKK9

Antitoxin

• AlphaFold DB: S6AY38