Detailed information of TA system TA186753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4062483..4063110
• Replicon: chromosome
• Accession: NZ_CP068148
• Organism: Serratia liquefaciens strain FDAARGOS_1081

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: I6I38_RS18960
• Protein ID: WP_197821222.1
• Coordinates: 4062889..4063110 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A379ZYL8
• Locus tag: I6I38_RS18955
• Protein ID: WP_020825515.1
• Coordinates: 4062483..4062851 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I38_RS18920	4058532..4058786	+	255	WP_020825521.1	type B 50S ribosomal protein L31	-
I6I38_RS18925	4058799..4058939	+	141	WP_012005563.1	type B 50S ribosomal protein L36	-
I6I38_RS18930	4059052..4059930	-	879	WP_020825520.1	metal ABC transporter substrate-binding protein	-
I6I38_RS18935	4059956..4060813	-	858	WP_041414279.1	metal ABC transporter permease	-
I6I38_RS18940	4060800..4061522	-	723	WP_201895666.1	ABC transporter ATP-binding protein	-
I6I38_RS18945	4061519..4061665	-	147	WP_020825517.1	hypothetical protein	-
I6I38_RS18950	4061994..4062320	+	327	WP_201896485.1	hypothetical protein	-
I6I38_RS18955	4062483..4062851	+	369	WP_020825515.1	Hha toxicity modulator TomB	Antitoxin
I6I38_RS18960	4062889..4063110	+	222	WP_197821222.1	hemolysin expression modulator Hha	Toxin
I6I38_RS18965	4063187..4064119	-	933	WP_130016230.1	LysR family transcriptional regulator	-
I6I38_RS18970	4064258..4066045	+	1788	WP_130016231.1	adenine deaminase	-
I6I38_RS18975	4066210..4067553	+	1344	WP_130016232.1	NCS2 family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4061967..4072166	10199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (14-70)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8727.21 Da
• Isoelectric Point: 7.9877

>T186753 WP_197821222.1 NZ_CP068148:4062889-4063110 [Serratia liquefaciens]
ITPKVIMTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Nucleotide

• Length: 222 bp

>T186753 NZ_CP089772:71859-72260 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14189.89 Da
• Isoelectric Point: 4.5140

>AT186753 WP_020825515.1 NZ_CP068148:4062483-4062851 [Serratia liquefaciens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEGICTTMKS

Nucleotide

• Length: 369 bp

>AT186753 NZ_CP089772:71627-71866 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A379ZYL8