Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2928989..2929607 Replicon chromosome
Accession NZ_CP068147
Organism Yersinia enterocolitica strain FDAARGOS_1090

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag I6I47_RS13050 Protein ID WP_002208622.1
Coordinates 2928989..2929192 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7U7FKE5
Locus tag I6I47_RS13055 Protein ID WP_005163463.1
Coordinates 2929239..2929607 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I47_RS13020 2924360..2924698 + 339 WP_002208627.1 P-II family nitrogen regulator -
I6I47_RS13025 2924738..2926027 + 1290 WP_049530802.1 ammonium transporter AmtB -
I6I47_RS13030 2926141..2927004 - 864 WP_005163474.1 acyl-CoA thioesterase II -
I6I47_RS13035 2927265..2927795 + 531 WP_020283453.1 YbaY family lipoprotein -
I6I47_RS13040 2927847..2928152 - 306 WP_005163466.1 MGMT family protein -
I6I47_RS13050 2928989..2929192 - 204 WP_002208622.1 expression modulating protein YmoA Toxin
I6I47_RS13055 2929239..2929607 - 369 WP_005163463.1 Hha toxicity modulator TomB Antitoxin
I6I47_RS13060 2929765..2930118 - 354 WP_005163460.1 hypothetical protein -
I6I47_RS13065 2930884..2931594 + 711 WP_013649453.1 ABC transporter ATP-binding protein -
I6I47_RS13070 2931608..2932465 + 858 WP_020283451.1 metal ABC transporter permease -
I6I47_RS13075 2932528..2933406 + 879 WP_013649455.1 metal ABC transporter substrate-binding protein -
I6I47_RS13080 2933534..2933677 - 144 WP_002208618.1 type B 50S ribosomal protein L36 -
I6I47_RS13085 2933693..2933953 - 261 WP_005163438.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T186727 WP_002208622.1 NZ_CP068147:c2929192-2928989 [Yersinia enterocolitica]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T186727 NZ_CP089767:c49641-49225 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14192.90 Da        Isoelectric Point: 4.3377

>AT186727 WP_005163463.1 NZ_CP068147:c2929607-2929239 [Yersinia enterocolitica]
MDEYSPKRHDVAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDDGDLSELVEEYL
DDTYTLFSSYGINDPELQRWQKTKERLFRLFSGEYICTLMKT

Download         Length: 369 bp

>AT186727 NZ_CP089767:c49868-49638 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
PDB 2MN2
AlphaFold DB A0A7U7FKE5

References