Detailed information of TA system TA186706

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 2038778..2039362
• Replicon: chromosome
• Accession: NZ_CP068146
• Organism: Yersinia enterocolitica strain FDAARGOS_1082

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7U7ISC5
• Locus tag: I6I39_RS08925
• Protein ID: WP_005165296.1
• Coordinates: 2038778..2039146 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6I39_RS08930
• Protein ID: WP_129019424.1
• Coordinates: 2039210..2039362 (-)
• Length: 51 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I39_RS08905	2033984..2034904	+	921	WP_005165286.1	ROK family protein	-
I6I39_RS08910	2034989..2035951	+	963	WP_005165289.1	LacI family transcriptional regulator	-
I6I39_RS08915	2035985..2036458	-	474	WP_005165291.1	GNAT family N-acetyltransferase	-
I6I39_RS08920	2037694..2038548	-	855	WP_005165294.1	DUF4942 domain-containing protein	-
I6I39_RS08925	2038778..2039146	-	369	WP_005165296.1	TA system toxin CbtA family protein	Toxin
I6I39_RS08930	2039210..2039362	-	153	WP_129019424.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6I39_RS08935	2039431..2040633	+	1203	WP_201887635.1	IS256 family transposase	-
I6I39_RS08940	2040808..2042271	+	1464	WP_005166182.1	alpha/beta hydrolase	-
I6I39_RS08945	2042504..2043910	-	1407	WP_050124520.1	filamentous hemagglutinin N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2039431..2040633	1202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (1-45)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13609.55 Da
• Isoelectric Point: 6.4731

>T186706 WP_005165296.1 NZ_CP068146:c2039146-2038778 [Yersinia enterocolitica]
MQTLPVTSKRAAYACLSPVIVWQSMLTYLLEQHYGLVLSDTEFSDDAVIHEHIDAGISLADALNFTVEKFDLARTDHRGF
SCQEQSPFITSIDILRARRATGLMTRMGYQTITSVIRGGKQA

Nucleotide

• Length: 369 bp

>T186706 NZ_CP089762:3429829-3430047 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 51 a.a.
• Molecular weight: 5593.44 Da
• Isoelectric Point: 4.5803

>AT186706 WP_129019424.1 NZ_CP068146:c2039362-2039210 [Yersinia enterocolitica]
MELMLLSGELNPRYQHCVTLYRNGLICEADSLGSQGYVYLAIYPTPQSIA

Nucleotide

• Length: 153 bp

>AT186706 NZ_CP089762:3429428-3429802 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U7ISC5