Detailed information of TA system TA186686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 676051..676580
• Replicon: chromosome
• Accession: NZ_CP068143
• Organism: Staphylococcus aureus strain FDAARGOS_1086

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I6I43_RS03335
• Protein ID: WP_000621175.1
• Coordinates: 676218..676580 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: I6I43_RS03330
• Protein ID: WP_000948331.1
• Coordinates: 676051..676221 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I43_RS03300	671087..671647	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
I6I43_RS03305	671856..672335	+	480	WP_001287079.1	hypothetical protein	-
I6I43_RS03310	672328..673911	+	1584	WP_045177766.1	PH domain-containing protein	-
I6I43_RS03315	673898..674389	+	492	WP_072353916.1	PH domain-containing protein	-
I6I43_RS03320	674393..674752	+	360	WP_000581197.1	holo-ACP synthase	-
I6I43_RS03325	674818..675966	+	1149	WP_001281154.1	alanine racemase	-
I6I43_RS03330	676051..676221	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
I6I43_RS03335	676218..676580	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6I43_RS03340	676929..677930	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
I6I43_RS03345	678049..678375	+	327	WP_001052491.1	anti-sigma factor antagonist	-
I6I43_RS03350	678377..678856	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
I6I43_RS03355	678831..679601	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T186686 WP_000621175.1 NZ_CP068143:676218-676580 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T186686 NZ_CP089742:1626475-1626762 [Melaminivora jejuensis]
ATGCCGATTGAGTCGCCAGCAGACAGGCTGGTGCGGCTGACCTGGACGCAGGCGGCCTGGGACGACTACGCCTATTGGCA
GGGCCAGGACAAAAAAACCCTGAAACGCATCAACCTGCTGATCCAGGATTGCCTGCGCCAACCGCTTTCCGGCATCGGCA
AACCTGAGCCGCTGAAAGAAAACCTGTATGGCTTTTGGTCACGGCGCATCGACGATACGAACAGGCTGGTGTATTGCGTG
GACGGGCAGGCGTTGGTGGTGATCGCCTGCCGCTATCACTATCGCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT186686 WP_000948331.1 NZ_CP068143:676051-676221 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT186686 NZ_CP089742:1626276-1626485 [Melaminivora jejuensis]
GTGGCTGGAGCCTTCGAGGATGCCGACTACACAGTCATCGTCCGGCGTGACGCGCCCGATGCCGTGGTCATGTCTCTGGA
CACTTTCAACAGCCTGATGGAGACCGTGCACCTGCTCAAGTCGCCCGCCAACGCCACCCATCTGGCGCGCTCGATCGCCC
AGTTGCGCGCAGGCCAGGTGCAGGAGCGGGATCTGGCAGATGCCGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1