Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3944037..3944679 Replicon chromosome
Accession NZ_CP068135
Organism Bacillus cereus strain FDAARGOS_1084

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag I6I41_RS19430 Protein ID WP_000635965.1
Coordinates 3944037..3944387 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag I6I41_RS19435 Protein ID WP_000004570.1
Coordinates 3944392..3944679 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I41_RS19415 3940973..3941431 - 459 WP_000344237.1 SprT family protein -
I6I41_RS19420 3941627..3941743 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
I6I41_RS19425 3941801..3943969 - 2169 WP_016124211.1 RNA-binding transcriptional accessory protein -
I6I41_RS19430 3944037..3944387 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
I6I41_RS19435 3944392..3944679 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
I6I41_RS19440 3944988..3946157 - 1170 WP_000390615.1 alanine racemase -
I6I41_RS19445 3946275..3947225 - 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
I6I41_RS19450 3947382..3947741 - 360 WP_000583415.1 holo-ACP synthase -
I6I41_RS19455 3947835..3948407 + 573 WP_000908523.1 rhomboid family intramembrane serine protease -
I6I41_RS19460 3948400..3949362 - 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T186655 WP_000635965.1 NZ_CP068135:c3944387-3944037 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T186655 NZ_CP089611:1960916-1961164 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT186655 WP_000004570.1 NZ_CP068135:c3944679-3944392 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT186655 NZ_CP089611:1960704-1960916 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References