Detailed information of TA system TA186654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 1797686..1798664
• Replicon: chromosome
• Accession: NZ_CP068135
• Organism: Bacillus cereus strain FDAARGOS_1084

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: I6I41_RS08925
• Protein ID: WP_000624977.1
• Coordinates: 1797927..1798664 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: I6I41_RS08920
• Protein ID: WP_000237818.1
• Coordinates: 1797686..1797814 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I41_RS08895	1793665..1794267	-	603	WP_000517265.1	hypothetical protein	-
I6I41_RS08900	1794293..1795102	-	810	WP_000664543.1	C39 family peptidase	-
I6I41_RS08905	1795348..1796817	-	1470	WP_000287543.1	beta-Ala-His dipeptidase	-
I6I41_RS08910	1797029..1797418	+	390	WP_000713865.1	YxeA family protein	-
I6I41_RS08915	1797437..1797613	-	177	WP_000852629.1	stage II sporulation protein SB	-
I6I41_RS08920	1797686..1797814	-	129	WP_000237818.1	hypothetical protein	Antitoxin
I6I41_RS08925	1797927..1798664	-	738	WP_000624977.1	stage II sporulation protein SA	Toxin
I6I41_RS08930	1798902..1799639	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
I6I41_RS08935	1799806..1800288	-	483	WP_000191886.1	MarR family winged helix-turn-helix transcriptional regulator	-
I6I41_RS08940	1800396..1802081	+	1686	WP_074619254.1	alpha-keto acid decarboxylase family protein	-
I6I41_RS08945	1802239..1803021	-	783	WP_074619255.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T186654 WP_000624977.1 NZ_CP068135:c1798664-1797927 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T186654 NZ_CP089611:c1940418-1940029 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT186654 WP_000237818.1 NZ_CP068135:c1797814-1797686 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT186654 NZ_CP089611:c1940642-1940415 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5