Detailed information of TA system TA186653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1361485..1362119
• Replicon: chromosome
• Accession: NZ_CP068128
• Organism: Enterococcus casseliflavus strain FDAARGOS_1121

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1I1S5M8
• Locus tag: I6I78_RS06375
• Protein ID: WP_074932270.1
• Coordinates: 1361769..1362119 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: I6I78_RS06370
• Protein ID: WP_201705862.1
• Coordinates: 1361485..1361775 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I78_RS06345	1357070..1357807	+	738	WP_060791770.1	tRNA pseudouridine(38-40) synthase TruA	-
I6I78_RS06350	1357850..1358608	+	759	WP_005234612.1	type I methionyl aminopeptidase	-
I6I78_RS06355	1358940..1359818	-	879	WP_201705860.1	DMT family transporter	-
I6I78_RS06360	1359815..1360300	-	486	WP_167822960.1	GNAT family N-acetyltransferase	-
I6I78_RS06365	1360406..1361275	+	870	WP_201705861.1	AraC family transcriptional regulator	-
I6I78_RS06370	1361485..1361775	+	291	WP_201705862.1	PbsX family transcriptional regulator	Antitoxin
I6I78_RS06375	1361769..1362119	+	351	WP_074932270.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6I78_RS06380	1362075..1362354	-	280	Protein_1226	helix-turn-helix domain-containing protein	-
I6I78_RS06385	1362430..1362861	-	432	WP_201705863.1	AsnC family transcriptional regulator	-
I6I78_RS06390	1362964..1363509	+	546	WP_005225439.1	cysteine hydrolase	-
I6I78_RS06395	1363826..1364749	+	924	WP_060791773.1	peptide-methionine (S)-S-oxide reductase MsrA	-
I6I78_RS06400	1365099..1366460	+	1362	WP_118214782.1	citrate transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (13-96)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13107.13 Da
• Isoelectric Point: 8.8234

>T186653 WP_074932270.1 NZ_CP068128:1361769-1362119 [Enterococcus casseliflavus]
MVRKPRQGDILLLDTAPRSGHEQTGKRPYIVLSHDMIADYSNVATVAPISSTSRNYPLYVSINPDYGMKTTGKVLLDQLT
TIDYEARACVFLEKAHEHLVDELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T186653 NZ_CP089611:1767272-1767676 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11251.86 Da
• Isoelectric Point: 4.9389

>AT186653 WP_201705862.1 NZ_CP068128:1361485-1361775 [Enterococcus casseliflavus]
MYIQKEVSNMEMLPIKKWGNSNGLRLPKYIMEYLGIHTDDKVKIIQEEVNGHKRLIIEAANLENELTIEELFENYTAEKN
HVEIQHLGEAVGNEKW

Nucleotide

• Length: 291 bp

>AT186653 NZ_CP089611:1767063-1767266 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• AlphaFold DB: A0A1I1S5M8