Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 30527..31367 Replicon plasmid unnamed1
Accession NZ_CP068124
Organism Enterococcus casseliflavus strain FDAARGOS_1122

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag I6I79_RS00180 Protein ID WP_201688343.1
Coordinates 30801..31367 (+) Length 189 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag I6I79_RS00175 Protein ID WP_000301765.1
Coordinates 30527..30799 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I79_RS00155 26204..27523 + 1320 WP_002338419.1 IS1380-like element ISSsu5 family transposase -
I6I79_RS00160 27738..29198 + 1461 Protein_31 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
I6I79_RS00165 29301..30197 + 897 WP_002387620.1 ParA family protein -
I6I79_RS00170 30302..30510 + 209 Protein_33 peptide-binding protein -
I6I79_RS00175 30527..30799 + 273 WP_000301765.1 antitoxin Antitoxin
I6I79_RS00180 30801..31367 + 567 WP_201688343.1 zeta toxin family protein Toxin
I6I79_RS00185 32051..32416 + 366 WP_201688348.1 hypothetical protein -
I6I79_RS00190 32950..33804 + 855 WP_201688344.1 DpnD/PcfM family protein -
I6I79_RS00195 33834..34091 - 258 WP_010717195.1 hypothetical protein -
I6I79_RS00200 34094..34393 - 300 WP_002325624.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / aac(6')-aph(2'') / ant(6)-Ia - 1..45244 45244
- flank IS/Tn ant(6)-Ia - 24544..27523 2979


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-182)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 189 a.a.        Molecular weight: 21121.97 Da        Isoelectric Point: 5.7365

>T186652 WP_201688343.1 NZ_CP068124:30801-31367 [Enterococcus casseliflavus]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKTYRPI

Download         Length: 567 bp

>T186652 NZ_CP089611:1687504-1687821 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT186652 WP_000301765.1 NZ_CP068124:30527-30799 [Enterococcus casseliflavus]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT186652 NZ_CP089611:1687205-1687507 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References