Detailed information of TA system TA186651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 22689..23796
• Replicon: plasmid unnamed1
• Accession: NZ_CP068124
• Organism: Enterococcus casseliflavus strain FDAARGOS_1122

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: I6I79_RS00135
• Protein ID: WP_000233000.1
• Coordinates: 22927..23796 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: I6I79_RS00130
• Protein ID: WP_201688342.1
• Coordinates: 22689..22937 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I79_RS00120	20566..20982	+	417	WP_000323438.1	recombinase	-
I6I79_RS00125	20984..22546	+	1563	WP_000136908.1	recombinase family protein	-
I6I79_RS00130	22689..22937	+	249	WP_201688342.1	helix-turn-helix transcriptional regulator	Antitoxin
I6I79_RS00135	22927..23796	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
I6I79_RS00140	23777..24511	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
I6I79_RS00145	24544..25407	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
I6I79_RS00150	25451..25977	+	527	Protein_29	adenine phosphoribosyltransferase	-
I6I79_RS00155	26204..27523	+	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / aac(6')-aph(2'') / ant(6)-Ia	-	1..45244	45244
	-	flank	IS/Tn	ant(6)-Ia	-	24544..27523	2979

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T186651 WP_000233000.1 NZ_CP068124:22927-23796 [Enterococcus casseliflavus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T186651 NZ_CP089611:c1575108-1574707 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9248.76 Da
• Isoelectric Point: 6.2725

>AT186651 WP_201688342.1 NZ_CP068124:22689-22937 [Enterococcus casseliflavus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKYLTNKGGCLY
GR

Nucleotide

• Length: 249 bp

>AT186651 NZ_CP089611:c1575362-1575105 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Toxin

• AlphaFold DB: R3JIN1