Detailed information of TA system TA186649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2465783..2466354
• Replicon: chromosome
• Accession: NZ_CP068118
• Organism: Enterococcus hirae strain FDAARGOS_1125

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I6I82_RS13010
• Protein ID: WP_135982259.1
• Coordinates: 2465783..2466124 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I6T4M0
• Locus tag: I6I82_RS13015
• Protein ID: WP_010738535.1
• Coordinates: 2466124..2466354 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I82_RS12990	2461006..2462007	+	1002	WP_034866081.1	aspartate-semialdehyde dehydrogenase	-
I6I82_RS12995	2462004..2463353	+	1350	WP_010720195.1	aspartate kinase	-
I6I82_RS13000	2463313..2464422	+	1110	WP_025480561.1	amidohydrolase	-
I6I82_RS13005	2464452..2465618	+	1167	WP_081123479.1	pyridoxal phosphate-dependent aminotransferase	-
I6I82_RS13010	2465783..2466124	-	342	WP_135982259.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6I82_RS13015	2466124..2466354	-	231	WP_010738535.1	hypothetical protein	Antitoxin
I6I82_RS13020	2466814..2468193	+	1380	WP_010738534.1	FAD-dependent oxidoreductase	-
I6I82_RS13025	2468373..2469338	-	966	WP_034866085.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13089.40 Da
• Isoelectric Point: 10.1962

>T186649 WP_135982259.1 NZ_CP068118:c2466124-2465783 [Enterococcus hirae]
VPKARNYIPKKGDIVWIDFDPSTGKEIQKRRPGLVVSRYEFNCTTMFAIICPITSTIKNLPTRYSLPKDLDTKGQVLTSQ
LKSLDFKERKLKKVENLPLQDMAKIDQIIQYIF

Nucleotide

• Length: 342 bp

>T186649 NZ_CP089611:c1394040-1393747 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8577.57 Da
• Isoelectric Point: 4.1196

>AT186649 WP_010738535.1 NZ_CP068118:c2466354-2466124 [Enterococcus hirae]
MLITKSRLQGSSVVITLPSDNGKKPSENQEYIVVYSEDGTITLVPKIEDPFSAGQEAEYYEKDEWKDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT186649 NZ_CP089611:c1394306-1394037 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A449E900