Detailed information of TA system TA186647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-Fic/Txe-HTH_24
• Location: 37380..38459
• Replicon: plasmid unnamed3
• Accession: NZ_CP068117
• Organism: Enterococcus hirae strain FDAARGOS_1125

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: I6I82_RS01160
• Protein ID: WP_135982191.1
• Coordinates: 37380..37649 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: I6I82_RS01165
• Protein ID: WP_135982193.1
• Coordinates: 37701..38459 (+)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I82_RS01130	33336..34544	+	1209	WP_135982174.1	DUF3991 and TOPRIM domain-containing protein	-
I6I82_RS01135	34555..35718	+	1164	WP_135982176.1	glucosaminidase domain-containing protein	-
I6I82_RS01140	35728..36204	+	477	WP_135982178.1	hypothetical protein	-
I6I82_RS01145	36405..36632	+	228	WP_135982185.1	hypothetical protein	-
I6I82_RS01150	36625..36963	+	339	WP_135982187.1	type II toxin-antitoxin system PemK/MazF family toxin	-
I6I82_RS01155	37141..37395	+	255	WP_135982189.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
I6I82_RS01160	37380..37649	+	270	WP_135982191.1	Txe/YoeB family addiction module toxin	Toxin
I6I82_RS01165	37701..38459	+	759	WP_135982193.1	Fic family protein	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..80453	80453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-84)

Antitoxin

• Fido domain (91-191)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10413.12 Da
• Isoelectric Point: 9.9106

>T186647 WP_135982191.1 NZ_CP068117:37380-37649 [Enterococcus hirae]
MGFTIEIKKNAKKDLPKLKAAGLHKKFDELIQVIRENPFQTPPPYEKLVGVPYYSRRINIQHRLVYSVDTEENVVVILSI
WSQYERGLN

Nucleotide

• Length: 270 bp

>T186647 NZ_CP089611:1244669-1245043 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 29414.21 Da
• Isoelectric Point: 4.6926

>AT186647 WP_135982193.1 NZ_CP068117:37701-38459 [Enterococcus hirae]
MTYNFYDEYSIARNQFHNQQFPSEYLDDILVRLAYHSSAIEGNTISLPQTVSIILHNTVPERTSLRELYEVENHRGAFAL
MLEKVQEKEKLSVSLIKDIHERLMDKLIIDRGRFKSNENAILGADFDTASPQETPLLMQQLVENLNFRLDNAKTEDEQLE
AILDSHIQFERIHPFSDGNGRTGRLVMNYSLLKEGFPPLVIQTEDKGQYLELLAEQNVRGLMKYALEQMAKEERRQEQFI
EKEKVQEHDLEL

Nucleotide

• Length: 759 bp

>AT186647 NZ_CP089611:1244475-1244672 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA