Detailed information of TA system TA186622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 19112..19665
• Replicon: chromosome
• Accession: NZ_CP068096
• Organism: Serratia plymuthica strain FDAARGOS_1138

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S4YH80
• Locus tag: I6I95_RS00090
• Protein ID: WP_020438747.1
• Coordinates: 19112..19426 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S4YG56
• Locus tag: I6I95_RS00095
• Protein ID: WP_020438746.1
• Coordinates: 19429..19665 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I95_RS00070	14880..16037	-	1158	WP_006324392.1	YbfB/YjiJ family MFS transporter	-
I6I95_RS00075	16145..17062	+	918	WP_004942342.1	LysR family transcriptional regulator	-
I6I95_RS00080	17091..18218	+	1128	WP_004942340.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
I6I95_RS00085	18233..19078	+	846	WP_006324390.1	S-formylglutathione hydrolase	-
I6I95_RS00090	19112..19426	-	315	WP_020438747.1	CcdB family protein	Toxin
I6I95_RS00095	19429..19665	-	237	WP_020438746.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
I6I95_RS00100	19810..20718	-	909	WP_004942335.1	glutathione ABC transporter permease GsiD	-
I6I95_RS00105	20728..21648	-	921	WP_006324386.1	glutathione ABC transporter permease GsiC	-
I6I95_RS00110	21701..23239	-	1539	WP_006324385.1	glutathione ABC transporter substrate-binding protein GsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11685.58 Da
• Isoelectric Point: 5.8036

>T186622 WP_020438747.1 NZ_CP068096:c19426-19112 [Serratia plymuthica]
MQFTVYANTGKSAVYPLLLDVTSDIIGQLNRRVVIPLLPIEKYPASTRPERLNPLVRLVDDNEYAVMTHEMASIPVRILG
AEFCDASQYRTKVKAAIDFLFDGI

Nucleotide

• Length: 315 bp

>T186622 NZ_CP089610:c3715975-3715547 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9078.16 Da
• Isoelectric Point: 6.7184

>AT186622 WP_020438746.1 NZ_CP068096:c19665-19429 [Serratia plymuthica]
MPVKQRTTQSVTMAVERSLLSRAREAGINLSATLNAALDAELRQHEMRKWKEENREAIDALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT186622 NZ_CP089610:c3716221-3715979 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: S4YH80

Antitoxin

• AlphaFold DB: S4YG56