Detailed information of TA system TA186621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 79527..80302
• Replicon: plasmid unnamed1
• Accession: NZ_CP068091
• Organism: Shigella boydii strain FDAARGOS_1139

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q31SE5
• Locus tag: I6I96_RS22715
• Protein ID: WP_000405246.1
• Coordinates: 79820..80302 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: I6I96_RS22710
• Protein ID: WP_010921625.1
• Coordinates: 79527..79829 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I96_RS22695	76201..77655	+	1455	WP_000701107.1	type 3 secretion system effector OspC2	-
I6I96_RS22700	77794..79002	-	1209	WP_000198550.1	IS91 family transposase	-
I6I96_RS22705	78983..79255	-	273	WP_000019158.1	hypothetical protein	-
I6I96_RS22710	79527..79829	+	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
I6I96_RS22715	79820..80302	+	483	WP_000405246.1	GNAT family N-acetyltransferase	Toxin
I6I96_RS22720	80370..80893	+	524	Protein_98	transposase	-
I6I96_RS22725	80936..81274	-	339	Protein_99	transposase	-
I6I96_RS22730	81348..82516	+	1169	WP_094096822.1	IS3 family transposase	-
I6I96_RS22735	82540..83232	-	693	WP_000625262.1	type III secretion system effector cysteine methyltransferase OspZ	-
I6I96_RS22740	83288..83440	+	153	Protein_102	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH1.4 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ospE2 / ipaH9.8 / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2	1..122799	122799
	-	inside	IScluster/Tn	-	ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2	74004..112811	38807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17748.55 Da
• Isoelectric Point: 7.0382

>T186621 WP_000405246.1 NZ_CP068091:79820-80302 [Shigella boydii]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCIQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T186621 NZ_CP089610:3561103-3561447 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT186621 WP_010921625.1 NZ_CP068091:79527-79829 [Shigella boydii]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT186621 NZ_CP089610:3561444-3561773 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: Q31SE5

Antitoxin

• AlphaFold DB: Q7BEG8