Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 60151..60776 Replicon plasmid unnamed1
Accession NZ_CP068091
Organism Shigella boydii strain FDAARGOS_1139

Toxin (Protein)


Gene name vapC Uniprot ID Q6XVU6
Locus tag I6I96_RS22600 Protein ID WP_000911318.1
Coordinates 60151..60549 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag I6I96_RS22605 Protein ID WP_000450530.1
Coordinates 60549..60776 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I96_RS22575 56116..57066 + 951 WP_005059240.1 virulence factor VirK -
I6I96_RS22580 57131..58075 + 945 WP_005027201.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
I6I96_RS22585 58229..58900 - 672 Protein_71 IS630 family transposase -
I6I96_RS22590 58916..59613 - 698 Protein_72 IS1 family transposase -
I6I96_RS22595 59669..60142 + 474 Protein_73 conjugal transfer protein TraD -
I6I96_RS22600 60151..60549 - 399 WP_000911318.1 tRNA(fMet)-specific endonuclease VapC Toxin
I6I96_RS22605 60549..60776 - 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
I6I96_RS22610 60861..61277 + 417 WP_171548060.1 conjugative relaxase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH1.4 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ospE2 / ipaH9.8 / ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospD2 / ospF / ospD1 / ipgB2 1..122799 122799
- inside IScluster/Tn - ospE2 / ipaH9.8 30781..59419 28638


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14845.09 Da        Isoelectric Point: 8.5264

>T186620 WP_000911318.1 NZ_CP068091:c60549-60151 [Shigella boydii]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T186620 NZ_CP089610:3192667-3193110 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT186620 WP_000450530.1 NZ_CP068091:c60776-60549 [Shigella boydii]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT186620 NZ_CP089610:3192423-3192680 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6SD6

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References