Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4158071..4158646 Replicon chromosome
Accession NZ_CP068090
Organism Shigella boydii strain FDAARGOS_1139

Toxin (Protein)


Gene name pemK Uniprot ID I6EL31
Locus tag I6I96_RS20790 Protein ID WP_000373726.1
Coordinates 4158329..4158646 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID I6EL32
Locus tag I6I96_RS20785 Protein ID WP_000288814.1
Coordinates 4158071..4158328 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I96_RS20760 4153137..4154305 + 1169 WP_094112657.1 IS3 family transposase -
I6I96_RS20765 4154705..4155070 + 366 WP_000253098.1 hypothetical protein -
I6I96_RS20770 4155083..4156687 + 1605 WP_000628841.1 hypothetical protein -
I6I96_RS20775 4157058..4157519 + 462 WP_000201268.1 hypothetical protein -
I6I96_RS20780 4157516..4157722 + 207 WP_000979502.1 helix-turn-helix domain-containing protein -
I6I96_RS20785 4158071..4158328 + 258 WP_000288814.1 antitoxin Antitoxin
I6I96_RS20790 4158329..4158646 + 318 WP_000373726.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I6I96_RS20795 4158806..4158952 - 147 WP_000285696.1 hypothetical protein -
I6I96_RS20800 4159511..4159915 + 405 Protein_4043 DNA-binding protein -
I6I96_RS20805 4159968..4161181 + 1214 WP_134810244.1 IS3 family transposase -
I6I96_RS20810 4161224..4161709 + 486 Protein_4045 integrase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4143520..4175131 31611


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-105)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11293.94 Da        Isoelectric Point: 10.2965

>T186610 WP_000373726.1 NZ_CP068090:4158329-4158646 [Shigella boydii]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVPVTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARL

Download         Length: 318 bp

>T186610 NZ_CP089610:2879951-2880328 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9280.76 Da        Isoelectric Point: 4.5821

>AT186610 WP_000288814.1 NZ_CP068090:4158071-4158328 [Shigella boydii]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT186610 NZ_CP089610:2879697-2879954 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T2UX62

Antitoxin

Source ID Structure
AlphaFold DB A0A085HLS8

References