Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1587488..1588142 Replicon chromosome
Accession NZ_CP068090
Organism Shigella boydii strain FDAARGOS_1139

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I6I96_RS07925 Protein ID WP_000244777.1
Coordinates 1587735..1588142 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6I96_RS07920 Protein ID WP_000354046.1
Coordinates 1587488..1587754 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I96_RS07895 1582657..1583400 + 744 Protein_1542 SDR family oxidoreductase -
I6I96_RS07900 1583457..1584890 - 1434 WP_039061725.1 6-phospho-beta-glucosidase BglA -
I6I96_RS07905 1584935..1585246 + 312 WP_001182983.1 N(4)-acetylcytidine aminohydrolase -
I6I96_RS07910 1585410..1586069 + 660 WP_000250272.1 hemolysin III family protein -
I6I96_RS07915 1586265..1587245 - 981 WP_000886094.1 tRNA-modifying protein YgfZ -
I6I96_RS07920 1587488..1587754 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6I96_RS07925 1587735..1588142 + 408 WP_000244777.1 toxin CptA Toxin
I6I96_RS07930 1588182..1588703 - 522 WP_001055874.1 flavodoxin FldB -
I6I96_RS07935 1588815..1589711 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6I96_RS07940 1589736..1590446 + 711 WP_000715205.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6I96_RS07945 1590452..1592185 + 1734 WP_000813210.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T186607 WP_000244777.1 NZ_CP068090:1587735-1588142 [Shigella boydii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T186607 NZ_CP089610:2819401-2819826 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT186607 WP_000354046.1 NZ_CP068090:1587488-1587754 [Shigella boydii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT186607 NZ_CP089610:2819174-2819395 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References