Detailed information of TA system TA186597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1208537..1209051
• Replicon: chromosome
• Accession: NZ_CP068073
• Organism: Staphylococcus condimenti strain FDAARGOS_1148

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I6J05_RS06160
• Protein ID: WP_082107871.1
• Coordinates: 1208537..1208887 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B9DMI8
• Locus tag: I6J05_RS06165
• Protein ID: WP_015900820.1
• Coordinates: 1208884..1209051 (-)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J05_RS06135	1204078..1204848	-	771	WP_047133044.1	RNA polymerase sigma factor SigB	-
I6J05_RS06140	1204823..1205302	-	480	WP_047133045.1	anti-sigma B factor RsbW	-
I6J05_RS06145	1205304..1205630	-	327	WP_015900817.1	anti-sigma factor antagonist	-
I6J05_RS06150	1205707..1206708	-	1002	WP_047133046.1	PP2C family protein-serine/threonine phosphatase	-
I6J05_RS06155	1206953..1208269	+	1317	WP_063164607.1	ISL3 family transposase	-
I6J05_RS06160	1208537..1208887	-	351	WP_082107871.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6J05_RS06165	1208884..1209051	-	168	WP_015900820.1	hypothetical protein	Antitoxin
I6J05_RS06170	1209132..1210286	-	1155	WP_047132378.1	alanine racemase	-
I6J05_RS06175	1210350..1210703	-	354	WP_047132377.1	holo-ACP synthase	-
I6J05_RS06180	1210705..1211190	-	486	WP_063164645.1	PH domain-containing protein	-
I6J05_RS06185	1211195..1212667	-	1473	WP_047132375.1	PH domain-containing protein	-
I6J05_RS06190	1212660..1213130	-	471	WP_047132374.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1206953..1208269	1316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13093.22 Da
• Isoelectric Point: 10.0708

>T186597 WP_082107871.1 NZ_CP068073:c1208887-1208537 [Staphylococcus condimenti]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAMTGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Nucleotide

• Length: 351 bp

>T186597 NZ_CP089610:c2199720-2199391 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6211.75 Da
• Isoelectric Point: 3.6836

>AT186597 WP_015900820.1 NZ_CP068073:c1209051-1208884 [Staphylococcus condimenti]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Nucleotide

• Length: 168 bp

>AT186597 NZ_CP089610:c2200094-2199717 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A380F5Y4