Detailed information of TA system TA186595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1658255..1658784
• Replicon: chromosome
• Accession: NZ_CP068069
• Organism: Staphylococcus equorum strain FDAARGOS_1149

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K7S376
• Locus tag: I6J06_RS08185
• Protein ID: WP_002511418.1
• Coordinates: 1658255..1658617 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J9GY23
• Locus tag: I6J06_RS08190
• Protein ID: WP_002507108.1
• Coordinates: 1658614..1658784 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J06_RS08165	1655260..1656030	-	771	WP_002507114.1	RNA polymerase sigma factor SigB	-
I6J06_RS08170	1656005..1656487	-	483	WP_002507113.1	anti-sigma B factor RsbW	-
I6J06_RS08175	1656489..1656815	-	327	WP_002511417.1	anti-sigma factor antagonist	-
I6J06_RS08180	1656895..1657896	-	1002	WP_002507111.1	PP2C family protein-serine/threonine phosphatase	-
I6J06_RS08185	1658255..1658617	-	363	WP_002511418.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6J06_RS08190	1658614..1658784	-	171	WP_002507108.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
I6J06_RS08195	1658872..1660020	-	1149	WP_021339950.1	alanine racemase	-
I6J06_RS08200	1660144..1660494	-	351	WP_021339949.1	holo-ACP synthase	-
I6J06_RS08205	1660527..1661063	-	537	WP_002507105.1	PH domain-containing protein	-
I6J06_RS08210	1661056..1662561	-	1506	WP_021339948.1	PH domain-containing protein	-
I6J06_RS08215	1662554..1663033	-	480	WP_021339947.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13432.59 Da
• Isoelectric Point: 10.2178

>T186595 WP_002511418.1 NZ_CP068069:c1658617-1658255 [Staphylococcus equorum]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSESKMKEVNVAIDISLGLHNVRNSKS

Nucleotide

• Length: 363 bp

>T186595 NZ_CP089610:c1946263-1945874 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6305.92 Da
• Isoelectric Point: 4.0121

>AT186595 WP_002507108.1 NZ_CP068069:c1658784-1658614 [Staphylococcus equorum]
MASFNQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYLSSKNNNE

Nucleotide

• Length: 171 bp

>AT186595 NZ_CP089610:c1946487-1946260 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Toxin

• AlphaFold DB: K7S376

Antitoxin

• AlphaFold DB: J9GY23