Detailed information of TA system TA186578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sezT-pezT/zeta-couple_hipB
• Location: 1050256..1051520
• Replicon: chromosome
• Accession: NZ_CP068060
• Organism: Streptococcus anginosus strain FDAARGOS_1155

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: I0SCS6
• Locus tag: I6J12_RS05030
• Protein ID: WP_003036796.1
• Coordinates: 1050256..1051044 (-)
• Length: 263 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: I6J12_RS05035
• Protein ID: WP_003024006.1
• Coordinates: 1051044..1051520 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J12_RS05005	1045288..1045647	-	360	WP_003036844.1	hypothetical protein	-
I6J12_RS05010	1046039..1046893	-	855	WP_003036855.1	hypothetical protein	-
I6J12_RS05015	1046900..1047364	-	465	WP_041791155.1	Mov34/MPN/PAD-1 family protein	-
I6J12_RS05020	1047342..1049108	-	1767	WP_003036645.1	ThiF family adenylyltransferase	-
I6J12_RS05025	1049117..1050153	-	1037	Protein_988	hypothetical protein	-
I6J12_RS05030	1050256..1051044	-	789	WP_003036796.1	zeta toxin family protein	Toxin
I6J12_RS05035	1051044..1051520	-	477	WP_003024006.1	helix-turn-helix domain-containing protein	Antitoxin
I6J12_RS05040	1051590..1051877	-	288	WP_003036759.1	hypothetical protein	-
I6J12_RS05045	1051921..1052310	-	390	WP_003036708.1	hypothetical protein	-
I6J12_RS05050	1052307..1052534	-	228	WP_003036737.1	hypothetical protein	-
I6J12_RS05055	1052589..1053677	-	1089	WP_003036783.1	toprim domain-containing protein	-
I6J12_RS05060	1053716..1054354	-	639	WP_003036847.1	hypothetical protein	-
I6J12_RS05065	1054494..1055492	+	999	WP_003036688.1	D-Ala D-Ala ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (31-217)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 263 a.a.
• Molecular weight: 30088.19 Da
• Isoelectric Point: 7.1606

>T186578 WP_003036796.1 NZ_CP068060:c1051044-1050256 [Streptococcus anginosus]
MRLEEFSEDEFQKALQRTIRSLTLTRGKTIPDQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLAL
QEKYGKDSVDYTKGFAGKMVEHLVDELSTQGYHLLIEGTLRTTQVPRKTAQLLRKKGYHISLAVIGTKPELSYLSTLIRY
EELYTINPNQARATPKEHHDGIVEHLVDNLRELENDKLFDRIQIYQRDRTCIYDSETDEISAATVLQECLFGKWSKVEEE
MLKVGQERLRDLCWKNGSSGIF

Nucleotide

• Length: 789 bp

>T186578 NZ_CP089610:719821-720216 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCCGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18260.64 Da
• Isoelectric Point: 4.3971

>AT186578 WP_003024006.1 NZ_CP068060:c1051520-1051044 [Streptococcus anginosus]
MIGDNIKSLRKTHDLTQPEFAKIVGISRNSLSRYENGTSSVSTELIDRICQKFNVSYVDIVGEDKMLTPLEDYQLTLKVE
VIKERGAAILSQLYRYQDSQDIAFDDETNPWILMSDDLAELIHTKIYLVDTFDEIERYNGYLDGIERMLDTVYHRVVA

Nucleotide

• Length: 477 bp

>AT186578 NZ_CP089610:719567-719821 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: I0SCS6