Detailed information of TA system TA186574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sezT-pezT/zeta-couple_hipB
• Location: 666636..667888
• Replicon: chromosome
• Accession: NZ_CP068057
• Organism: Streptococcus dysgalactiae strain FDAARGOS_1157

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: -
• Locus tag: I6J14_RS03250
• Protein ID: WP_115252829.1
• Coordinates: 666636..667412 (-)
• Length: 259 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: I6J14_RS03255
• Protein ID: WP_000578851.1
• Coordinates: 667412..667888 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J14_RS03235	661975..662334	-	360	WP_000529540.1	hypothetical protein	-
I6J14_RS03240	662763..665846	-	3084	WP_115276397.1	DEAD/DEAH box helicase	-
I6J14_RS03245	665843..666652	-	810	WP_032459422.1	DUF1837 domain-containing protein	-
I6J14_RS03250	666636..667412	-	777	WP_115252829.1	zeta toxin family protein	Toxin
I6J14_RS03255	667412..667888	-	477	WP_000578851.1	helix-turn-helix domain-containing protein	Antitoxin
I6J14_RS03260	667959..668246	-	288	WP_000765214.1	hypothetical protein	-
I6J14_RS03265	668290..668685	-	396	WP_000159225.1	hypothetical protein	-
I6J14_RS03270	668689..668916	-	228	WP_115276396.1	hypothetical protein	-
I6J14_RS03275	668970..670058	-	1089	WP_000196746.1	toprim domain-containing protein	-
I6J14_RS03280	670097..670735	-	639	WP_000932871.1	hypothetical protein	-
I6J14_RS03285	670846..671136	-	291	WP_000893078.1	hypothetical protein	-
I6J14_RS03290	671151..671450	-	300	WP_000195241.1	hypothetical protein	-
I6J14_RS03295	671520..672329	-	810	WP_000200962.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	490339..705940	215601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 259 a.a.
• Molecular weight: 29508.28 Da
• Isoelectric Point: 6.5044

>T186574 WP_115252829.1 NZ_CP068057:c667412-666636 [Streptococcus dysgalactiae]
MALADYHQDDFDFALKRTIRSLTRGKETSVNPNAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSYRSLHPNYLALQE
EYSKDSVDYTKGFAGKMVEHLVDELSKQGYHLLIEGTLRTAEVRRKTAQLLKSRGYQVSLALIATKPELSYLSTLIRYEE
VYAVNPNQARATPKEHHDAIVEHLVDNLRELETDKLFDQIQIYQRDRRCVYDSETDKGSAADVLQECLFGKWSKVEEEMM
KVGQEQLREVGRRNEKDA

Nucleotide

• Length: 777 bp

>T186574 NZ_CP089610:c698388-697996 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18088.56 Da
• Isoelectric Point: 4.4289

>AT186574 WP_000578851.1 NZ_CP068057:c667888-667412 [Streptococcus dysgalactiae]
MIGENIKSLRKTHDLTQPEFAKIVGISRNSLSRYENGTSSVTTELIDCICQKFNVSYVDIVGEEKMLTPVEDYQLTLKIE
VIKERGAAILSQLYRYQDSQGIAFDDETNPWILMSDDLSDLINTKIYLVNTFDEVERYNGYLDGIERMLVLARHQVVA

Nucleotide

• Length: 477 bp

>AT186574 NZ_CP089610:c698642-698385 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA