Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 2138882..2139515 Replicon chromosome
Accession NZ_CP068054
Organism Weizmannia coagulans strain FDAARGOS_1160

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag I6J17_RS10485 Protein ID WP_013860680.1
Coordinates 2139165..2139515 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag I6J17_RS10480 Protein ID WP_014096808.1
Coordinates 2138882..2139160 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J17_RS10460 2134988..2135587 - 600 WP_029142791.1 rhomboid family intramembrane serine protease -
I6J17_RS10465 2135869..2136219 + 351 WP_029142790.1 holo-ACP synthase -
I6J17_RS10470 2136360..2137376 + 1017 WP_013860683.1 outer membrane lipoprotein carrier protein LolA -
I6J17_RS10475 2137578..2138729 + 1152 WP_029142789.1 alanine racemase -
I6J17_RS10480 2138882..2139160 + 279 WP_014096808.1 hypothetical protein Antitoxin
I6J17_RS10485 2139165..2139515 + 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I6J17_RS10490 2140083..2140910 + 828 WP_029142788.1 RsbT co-antagonist protein RsbRA -
I6J17_RS10495 2140914..2141270 + 357 WP_017553290.1 STAS domain-containing protein -
I6J17_RS10500 2141275..2141676 + 402 WP_029142787.1 anti-sigma regulatory factor -
I6J17_RS10505 2141691..2142701 + 1011 WP_029142786.1 PP2C family protein-serine/threonine phosphatase -
I6J17_RS10510 2142770..2143099 + 330 WP_013860674.1 anti-sigma factor antagonist -
I6J17_RS10515 2143103..2143573 + 471 WP_017553287.1 anti-sigma B factor RsbW -
I6J17_RS10520 2143551..2144330 + 780 WP_029142785.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T186573 WP_013860680.1 NZ_CP068054:2139165-2139515 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T186573 NZ_CP089610:681079-681486 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT186573 WP_014096808.1 NZ_CP068054:2138882-2139160 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT186573 NZ_CP089610:680867-681082 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References