Detailed information of TA system TA186567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1790530..1792373
• Replicon: chromosome
• Accession: NZ_CP068050
• Organism: Burkholderia gladioli strain BBB-01

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: JKG63_RS26160
• Protein ID: WP_105850547.1
• Coordinates: 1791408..1792373 (-)
• Length: 322 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: JKG63_RS26155
• Protein ID: WP_044275309.1
• Coordinates: 1790530..1791390 (-)
• Length: 287 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKG63_RS26135	1785677..1787167	-	1491	WP_046574265.1	aldehyde dehydrogenase	-
JKG63_RS26140	1787244..1788077	-	834	WP_186028167.1	aspartate dehydrogenase	-
JKG63_RS26145	1788070..1789743	-	1674	WP_013689910.1	thiamine pyrophosphate-binding protein	-
JKG63_RS26150	1789736..1790533	-	798	WP_096750801.1	SDR family oxidoreductase	-
JKG63_RS26155	1790530..1791390	-	861	WP_044275309.1	IclR family transcriptional regulator	Antitoxin
JKG63_RS26160	1791408..1792373	-	966	WP_105850547.1	alpha/beta fold hydrolase	Toxin
JKG63_RS26165	1792400..1792927	-	528	WP_036038832.1	cupin domain-containing protein	-
JKG63_RS26170	1793026..1793796	-	771	WP_105850498.1	SDR family oxidoreductase	-
JKG63_RS26175	1793789..1794898	-	1110	WP_096750800.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
JKG63_RS26180	1794940..1795251	-	312	WP_025097963.1	hypothetical protein	-
JKG63_RS26185	1795254..1795571	-	318	WP_013689902.1	non-heme iron oxygenase ferredoxin subunit	-
JKG63_RS26190	1795987..1797066	+	1080	WP_025097962.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (66-305)

Antitoxin

• Transcription regulator IclR, N-terminal (25-73)

Sequences

Toxin

Protein

• Length: 322 a.a.
• Molecular weight: 32925.41 Da
• Isoelectric Point: 7.0329

>T186567 WP_105850547.1 NZ_CP068050:c1792373-1791408 [Burkholderia gladioli]
MSEIDPRGAEAEAGPDAALAARLWQFPARRIAIREAGLIGWRESGPAQGAGDSTARAAAGERALPVVLLHGIGSGAASWL
AQLDTLGASRRVLAWDAPGYGESTPVAPASPLAADYAASLAGWLDALAIERCVLVGHSLGAIVAGAFARRHAGRLAGLLL
VSPAAGYGAAPAATRDERRDARLAMLAALGPAGLAEQRSANMLSAHAGEAARAWVRRVMAQVKPAGYTQATHLLANADLA
ADLAAWRAMTDAAGAARVEVMVGAEDGITPPNACERIAAAAGVALRIVPRAGHAGYVEAPERYSELIGAFCARCDESWRQ
A

Nucleotide

• Length: 966 bp

>T186567 NZ_CP089607:302629-302985 [Rhodococcus qingshengii]
ATGATTCGACGCGCACACATTCACTGGGTGGATCTCGATCCGGCGCAGGGCAGTGAAGCGGCGAAACGACGACCGGCGGT
GGTGGTGAGCAACGACCGCGCCAACGCTACTGCAGAAAATCTCGGCAGGGGCGTGATCACCGTGGTGCCGTTGACCACCA
ATACTGCGCACGTGTTTCCGTTTCAGGTGATTGTCGAGTCCGACGAGTCAGGTTTGGCGCGAGCGTCGAAAGCGCAAGCG
GAGCAAGTGCGGTCGGTATCGGTTCGGCGTCTGGATCCTGAACCTGTCGGCAAGGTCGGGATGGAGACTTTCGCCGCACT
CGAGGACGCGCTGCGGCTGCACCTCGATCTGCAGTAA

Antitoxin

Protein

• Length: 287 a.a.
• Molecular weight: 31049.35 Da
• Isoelectric Point: 6.5132

>AT186567 WP_044275309.1 NZ_CP068050:c1791390-1790530 [Burkholderia gladioli]
MRSEPADSGAPEADAANGAYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNY
RLGVAVLRLGFEYLSSLELTDLGLPVIEGLREATGFTTHIVIRDGRDVVFVAKAQSAAPAFSSIRVNVGTRLPAHATTHG
HVLMGDLSEAELRALYPERQLERATRATPDSLEALLALVREDAARGYGISNSSFERGISVVTAPVRNDTQRIVAAVTVTV
PKPEIDAALIADGLVEKVVRAANELSRRLNYRSDDENSYLKALGLR

Nucleotide

• Length: 861 bp

>AT186567 NZ_CP089607:302420-302632 [Rhodococcus qingshengii]
ATGACCACGAAATTTACCATAAGTATCCCTGACGAGGATGCGCACCTGCTCGACGAGTATGTGCGTGATCACGGACTTCG
TGGCCGGTCGGCAGGAATTCGAGCCGCGCTTCGCCTACTCTCGACGTCCGGACTCGAACGTGACTACGCTGCAGCCTTTG
CTGAAGGTGACGTAGATGAAGTGTGGGACGCTGTCACGGCAGACACGGAATGA