Detailed information of TA system TA186565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 652344..652986
• Replicon: chromosome
• Accession: NZ_CP068050
• Organism: Burkholderia gladioli strain BBB-01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F2LQ48
• Locus tag: JKG63_RS21580
• Protein ID: WP_013690721.1
• Coordinates: 652564..652986 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JKG63_RS21575
• Protein ID: WP_123038586.1
• Coordinates: 652344..652577 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKG63_RS21545	647990..648307	+	318	WP_186172569.1	hypothetical protein	-
JKG63_RS21550	648529..648756	+	228	WP_172878703.1	hypothetical protein	-
JKG63_RS21555	648749..648994	+	246	WP_201446961.1	hypothetical protein	-
JKG63_RS21560	649073..650209	-	1137	WP_036055804.1	glutathione-independent formaldehyde dehydrogenase	-
JKG63_RS21565	650428..651462	-	1035	WP_186172620.1	flavodoxin family protein	-
JKG63_RS21570	651666..652010	+	345	WP_201446962.1	hypothetical protein	-
JKG63_RS21575	652344..652577	+	234	WP_123038586.1	DNA-binding protein	Antitoxin
JKG63_RS21580	652564..652986	+	423	WP_013690721.1	type II toxin-antitoxin system VapC family toxin	Toxin
JKG63_RS21585	653108..653788	-	681	WP_036055817.1	deoxyribose-phosphate aldolase	-
JKG63_RS21590	653938..654708	+	771	WP_025101959.1	DNA-binding transcriptional repressor DeoR	-
JKG63_RS21595	654728..655810	-	1083	WP_201446963.1	aldose 1-epimerase family protein	-
JKG63_RS21600	655813..657186	-	1374	WP_186034789.1	L-fucose:H+ symporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15649.91 Da
• Isoelectric Point: 8.5438

>T186565 WP_013690721.1 NZ_CP068050:652564-652986 [Burkholderia gladioli]
MYLIDTNVVSEVRKRDRADKGVMAFFRQAARDDAGLYLSVVTVGELRRGVEIIRHRGDESQATRLANWLDGVLREFAANI
LVVDKEIGQMWGYLRVPRSEHSLDKVIAATALIHDLTVVTRNVDDFAGTGARVLNPFKGP

Nucleotide

• Length: 423 bp

>T186565 NZ_CP089607:c110264-109962 [Rhodococcus qingshengii]
GTGCGCCCGATCCACGACGCGCAGCTCGGAGATCTTTGCCCGGTGTTGGTTCTGACGAGGGAGCTGGTTCGGCCTCATCT
GGGGACGGTCACTGTTGCGCCGATTCGTGCGGAGGGGCGGGGGTTGTCGACCGAGCTGAGTTTGGGCGAAGGCAACGGTG
TCCAAGATGGGTCTGTTGTCGACTGCGACGCCATAACGACGATTGCCGTGTCGGCACTCGGCGCGAACCGAGGATTTCTC
CTGCCGGCTCAGGAAGCATTGTTGAGCAGGGCGCTGCGATCAGCATTCGATCTCGAGTCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8542.65 Da
• Isoelectric Point: 5.0618

>AT186565 WP_123038586.1 NZ_CP068050:652344-652577 [Burkholderia gladioli]
MANLLVRNVDEMLVQILRERAAAHGHSAEAEHREILARALREPQRKTFAQVLMGMPNVGSDADFARVDDGEAANVFD

Nucleotide

• Length: 234 bp

>AT186565 NZ_CP089607:c110480-110265 [Rhodococcus qingshengii]
ATGAGTACGCAGATCGCGGTACGGCTACCCGAGGATATCGTGGCGTTTCTCGACAGTGAGGTCAGTGGCGGGCGCGCGAA
AAGCCGTGCCCAAGTCGTACTTCGAGCGTTGGAACGTGAACAGCGTCGACAGTTGGCGGTCCGTGACGTGGAGATCCTGT
CTGACGGCGCGGACAGCGATGTGAATATCGCTGCCGCGCACGGGAAGAAGAACTGA

Structures

Toxin

• AlphaFold DB: A0A833Q1H0