Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1988..2535 Replicon plasmid pXJW9B277-I1
Accession NZ_CP068044
Organism Escherichia coli strain XJW9B277

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag JJE78_RS25585 Protein ID WP_001384452.1
Coordinates 2257..2535 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID W0FTF6
Locus tag JJE78_RS25580 Protein ID WP_000079942.1
Coordinates 1988..2257 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJE78_RS25575 (46) 46..1077 + 1032 WP_000907870.1 plasmid replication initiator RepA -
JJE78_RS25580 (1988) 1988..2257 + 270 WP_000079942.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
JJE78_RS25585 (2257) 2257..2535 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
JJE78_RS25590 (2581) 2581..2793 + 213 Protein_3 3'-5' exonuclease -
JJE78_RS25595 (3349) 3349..3771 - 423 Protein_4 serine hydrolase -
JJE78_RS25600 (3818) 3818..4522 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JJE78_RS25605 (4802) 4802..5662 + 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
JJE78_RS25610 (5832) 5832..6587 + 756 WP_012372818.1 16S rRNA (guanine(1405)-N(7))-methyltransferase RmtB1 -
JJE78_RS25615 (6668) 6668..7216 - 549 WP_014342204.1 cation:proton antiporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / rmtB / qepA1 - 1..103093 103093


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T186558 WP_001384452.1 NZ_CP068044:2257-2535 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T186558 NZ_CP089600:c6137-5832 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10282.61 Da        Isoelectric Point: 7.2057

>AT186558 WP_000079942.1 NZ_CP068044:1988-2257 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT186558 NZ_CP089600:c6357-6139 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A140JYS5

References