Detailed information of TA system TA186554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 75710..76335
• Replicon: plasmid pXJW9B277-F18
• Accession: NZ_CP068043
• Organism: Escherichia coli strain XJW9B277

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JJE78_RS25240
• Protein ID: WP_000911313.1
• Coordinates: 75937..76335 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: JJE78_RS25235
• Protein ID: WP_000450520.1
• Coordinates: 75710..75937 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJE78_RS25235 (75710)	75710..75937	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JJE78_RS25240 (75937)	75937..76335	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
JJE78_RS25245 (76344)	76344..78497	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
JJE78_RS25250 (78593)	78593..79270	+	678	WP_048217701.1	IS66-like element accessory protein TnpA	-
JJE78_RS25255 (79270)	79270..79617	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
JJE78_RS25260 (79637)	79637..81208	+	1572	WP_000381395.1	IS66-like element ISCro1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / floR / sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-14 / fosA3 / tet(A) / qacE / aadA2 / dfrA12 / blaTEM-1B / sitABCD	iutA / iucD / iucC / iucB / iucA	1..133299	133299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T186554 WP_000911313.1 NZ_CP068043:75937-76335 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T186554 NZ_CP089598:c5439988-5439605 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT186554 WP_000450520.1 NZ_CP068043:75710-75937 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT186554 NZ_CP089598:c5440230-5439988 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2