Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 88501..90124 Replicon plasmid pXJW9B277-HI2-N
Accession NZ_CP068042
Organism Escherichia coli strain XJW9B277

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag JJE78_RS23775 Protein ID WP_000088046.1
Coordinates 88799..90124 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag JJE78_RS23770 Protein ID WP_024136327.1
Coordinates 88501..88794 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJE78_RS23745 (JJE78_23630) 84373..85614 + 1242 WP_001053338.1 VWA domain-containing protein -
JJE78_RS26590 85705..85836 + 132 WP_000374059.1 hypothetical protein -
JJE78_RS23750 (JJE78_23635) 86177..86458 - 282 WP_000077926.1 hypothetical protein -
JJE78_RS23755 (JJE78_23640) 86508..86699 - 192 WP_000398480.1 hypothetical protein -
JJE78_RS23760 (JJE78_23645) 86791..87132 - 342 WP_001151575.1 hypothetical protein -
JJE78_RS23765 (JJE78_23650) 87505..87897 + 393 WP_000341066.1 hypothetical protein -
JJE78_RS23770 (JJE78_23655) 88501..88794 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
JJE78_RS23775 (JJE78_23660) 88799..90124 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
JJE78_RS23780 (JJE78_23665) 90185..90391 + 207 WP_000134171.1 hypothetical protein -
JJE78_RS23785 (JJE78_23670) 90493..90903 + 411 WP_000985911.1 hypothetical protein -
JJE78_RS23790 (JJE78_23675) 90916..91731 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
JJE78_RS23795 (JJE78_23680) 91985..92410 + 426 WP_001043843.1 hypothetical protein -
JJE78_RS23800 (JJE78_23685) 92959..93267 + 309 WP_001224687.1 hypothetical protein -
JJE78_RS23805 (JJE78_23690) 93283..94140 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
JJE78_RS23810 (JJE78_23695) 94202..94405 + 204 WP_001194555.1 hypothetical protein -
JJE78_RS23815 (JJE78_23700) 94747..95103 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / floR / tet(A) / sul3 / lnu(F) / ant(3'')-Ia / aac(3)-IId / blaLAP-2 / blaTEM-1B / mph(A) / qnrS1 / blaCTX-M-55 / ARR-3 / dfrA14 - 1..270816 270816


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T186549 WP_000088046.1 NZ_CP068042:88799-90124 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T186549 NZ_CP089598:2072007-2072109 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT186549 WP_024136327.1 NZ_CP068042:88501-88794 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT186549 NZ_CP089598:c2072116-2071971 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References