Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3854639..3855293 Replicon chromosome
Accession NZ_CP068041
Organism Escherichia coli strain XJW9B277

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JJE78_RS18595 Protein ID WP_000244781.1
Coordinates 3854639..3855046 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJE78_RS18600 Protein ID WP_000354046.1
Coordinates 3855027..3855293 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJE78_RS18575 (3850596) 3850596..3852329 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
JJE78_RS18580 (3852335) 3852335..3853045 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJE78_RS18585 (3853070) 3853070..3853966 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JJE78_RS18590 (3854078) 3854078..3854599 + 522 WP_001055874.1 flavodoxin FldB -
JJE78_RS18595 (3854639) 3854639..3855046 - 408 WP_000244781.1 protein YgfX Toxin
JJE78_RS18600 (3855027) 3855027..3855293 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJE78_RS18605 (3855536) 3855536..3856516 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
JJE78_RS18610 (3856712) 3856712..3857371 - 660 WP_000250274.1 hemolysin III family protein -
JJE78_RS18615 (3857535) 3857535..3857846 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JJE78_RS18620 (3857891) 3857891..3859324 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JJE78_RS18625 (3859381) 3859381..3860124 - 744 WP_061359834.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T186543 WP_000244781.1 NZ_CP068041:c3855046-3854639 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T186543 NZ_CP089592:c2224562-2224386 [Bacillus subtilis]
GTGCTTGAGAAAATGGGTATCGTAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT186543 WP_000354046.1 NZ_CP068041:c3855293-3855027 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT186543 NZ_CP089592:2224503-2224603 [Bacillus subtilis]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTACGATACCCATTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References