Detailed information of TA system TA186540

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2963736..2964567
• Replicon: chromosome
• Accession: NZ_CP068041
• Organism: Escherichia coli strain XJW9B277

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JJE78_RS14515
• Protein ID: WP_000854814.1
• Coordinates: 2964193..2964567 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: JJE78_RS14510
• Protein ID: WP_001285585.1
• Coordinates: 2963736..2964104 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJE78_RS14475 (2958968)	2958968..2960038	+	1071	WP_000102644.1	patatin-like phospholipase family protein	-
JJE78_RS14480 (2960174)	2960174..2960857	+	684	WP_000775500.1	hypothetical protein	-
JJE78_RS14485 (2960873)	2960873..2961283	+	411	WP_000846713.1	hypothetical protein	-
JJE78_RS14490 (2961504)	2961504..2962325	+	822	WP_048217813.1	DUF932 domain-containing protein	-
JJE78_RS14495 (2962407)	2962407..2962886	+	480	WP_000860081.1	antirestriction protein	-
JJE78_RS14500 (2962902)	2962902..2963378	+	477	WP_001186773.1	RadC family protein	-
JJE78_RS14505 (2963441)	2963441..2963662	+	222	WP_000692323.1	DUF987 domain-containing protein	-
JJE78_RS14510 (2963736)	2963736..2964104	+	369	WP_001285585.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
JJE78_RS14515 (2964193)	2964193..2964567	+	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
JJE78_RS14520 (2964564)	2964564..2964758	+	195	WP_000988600.1	DUF5983 family protein	-
JJE78_RS14525 (2964804)	2964804..2964884	+	81	Protein_2879	hypothetical protein	-
JJE78_RS14530 (2965173)	2965173..2965301	-	129	Protein_2880	transposase domain-containing protein	-
JJE78_RS14535 (2965421)	2965421..2965555	+	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
JJE78_RS14540 (2965656)	2965656..2965985	-	330	WP_000450409.1	DUF496 family protein	-
JJE78_RS14545 (2966157)	2966157..2967215	-	1059	WP_001200890.1	FUSC family protein	-
JJE78_RS14550 (2967413)	2967413..2967886	-	474	WP_001105416.1	DNA gyrase inhibitor SbmC	-
JJE78_RS14555 (2968005)	2968005..2969171	-	1167	WP_000830144.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T186540 WP_000854814.1 NZ_CP068041:2964193-2964567 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T186540 NZ_CP089592:2074484-2074576 [Bacillus subtilis]
ATGTCAACATTCCAGGCATTAATGCTAATGCTTGCAATCGGGTCGTTTATAATCGCCCTATTGACGTATATAGAAAAAAT
AGACCTCCCTTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT186540 WP_001285585.1 NZ_CP068041:2963736-2964104 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT186540 NZ_CP089592:c2074730-2074557 [Bacillus subtilis]
TTACAATACATTACAGTGCATTTCTTTAATGAGAAATGCATAAAATAAAAAAGACCGAGGTGCTACCAACACCCCGGCTC
TGTACAAAAAGTTGCCCATCAAAGGGCTTGCTCGATGTGGTTCTAGGTAGGCCTATCCCTTCAGGTTCTGAGGCTCAAGG
GAGGTCTATTTTTT

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60