Detailed information of TA system TA186530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2283465..2284103
• Replicon: chromosome
• Accession: NZ_CP068041
• Organism: Escherichia coli strain XJW9B277

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: JJE78_RS11135
• Protein ID: WP_001447010.1
• Coordinates: 2283465..2283641 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JJE78_RS11140
• Protein ID: WP_001270286.1
• Coordinates: 2283687..2284103 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJE78_RS11115 (2279084)	2279084..2280259	-	1176	WP_001236265.1	BenE family transporter YdcO	-
JJE78_RS11120 (2280351)	2280351..2280887	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JJE78_RS11125 (2280960)	2280960..2282921	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JJE78_RS11130 (2283013)	2283013..2283243	-	231	WP_000494244.1	YncJ family protein	-
JJE78_RS11135 (2283465)	2283465..2283641	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JJE78_RS11140 (2283687)	2283687..2284103	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JJE78_RS11145 (2284182)	2284182..2285588	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
JJE78_RS11150 (2285833)	2285833..2286978	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
JJE78_RS11155 (2286996)	2286996..2288009	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
JJE78_RS11160 (2288010)	2288010..2288951	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T186530 WP_001447010.1 NZ_CP068041:2283465-2283641 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T186530 NZ_CP089586:2323385-2323651 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT186530 WP_001270286.1 NZ_CP068041:2283687-2284103 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT186530 NZ_CP089586:2323128-2323385 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8