Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1154851..1155469 Replicon chromosome
Accession NZ_CP068041
Organism Escherichia coli strain XJW9B277

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JJE78_RS05540 Protein ID WP_001291435.1
Coordinates 1154851..1155069 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JJE78_RS05545 Protein ID WP_000344800.1
Coordinates 1155095..1155469 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJE78_RS05505 (1150140) 1150140..1150712 + 573 WP_000779831.1 YbaY family lipoprotein -
JJE78_RS05510 (1150743) 1150743..1151054 - 312 WP_000409911.1 MGMT family protein -
JJE78_RS05520 (1151433) 1151433..1151786 + 354 WP_000878140.1 DUF1428 domain-containing protein -
JJE78_RS05525 (1151828) 1151828..1153378 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JJE78_RS05530 (1153542) 1153542..1154012 - 471 WP_000136192.1 YlaC family protein -
JJE78_RS05535 (1154128) 1154128..1154679 - 552 WP_000102564.1 maltose O-acetyltransferase -
JJE78_RS05540 (1154851) 1154851..1155069 - 219 WP_001291435.1 HHA domain-containing protein Toxin
JJE78_RS05545 (1155095) 1155095..1155469 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JJE78_RS05550 (1156015) 1156015..1159164 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JJE78_RS05555 (1159187) 1159187..1160380 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T186529 WP_001291435.1 NZ_CP068041:c1155069-1154851 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T186529 NZ_CP089586:1202084-1202272 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT186529 WP_000344800.1 NZ_CP068041:c1155469-1155095 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT186529 NZ_CP089586:1201795-1202055 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References