Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 60562..61121 Replicon plasmid pU90-5
Accession NZ_CP068040
Organism Escherichia coli strain U90

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag JKF55_RS26330 Protein ID WP_000421257.1
Coordinates 60562..60837 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag JKF55_RS26335 Protein ID WP_001178089.1
Coordinates 60837..61121 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF55_RS26310 56897..57262 + 366 WP_000124098.1 hypothetical protein -
JKF55_RS26315 57262..58449 + 1188 WP_116794643.1 IS91-like element IS91 family transposase -
JKF55_RS26320 58726..59091 + 366 WP_000091308.1 hypothetical protein -
JKF55_RS26325 59091..60278 + 1188 WP_201448040.1 IS91 family transposase -
JKF55_RS26330 60562..60837 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKF55_RS26335 60837..61121 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
JKF55_RS26340 62031..62888 - 858 WP_024233440.1 incFII family plasmid replication initiator RepA -
JKF55_RS26345 62881..62955 - 75 WP_001365705.1 RepA leader peptide Tap -
JKF55_RS26350 63192..63446 - 255 WP_000083830.1 replication regulatory protein RepA -
JKF55_RS26355 63711..63941 - 231 WP_001736714.1 hypothetical protein -
JKF55_RS26360 64105..64704 - 600 WP_063082122.1 hypothetical protein -
JKF55_RS26365 65091..65291 - 201 WP_015059022.1 hypothetical protein -
JKF55_RS26370 65423..65983 - 561 WP_000139328.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa / hlyC / hlyA / hlyB / hlyD 1..126454 126454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T186521 WP_000421257.1 NZ_CP068040:c60837-60562 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T186521 NZ_CP089584:c1076407-1076150 [Xanthomonas translucens pv. undulosa]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAAACCGACAAGAAGATGCTCAAGCGCGT
CAACGATCTGATCAAAGCCATTCAACGCGATCCCTTCCAAGGCGTCGGCAAGCCAGAACCGCTGCGCCATGCCTTGGCCG
GCTACTGGTCGCGGCGCATCAACGAAGAACACCGCATCGTGTACAGGGTCGAGGCGGGCATCCTGCTGATCGCGCAGGTG
CGCTACCACTATCTGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10456.86 Da        Isoelectric Point: 5.6558

>AT186521 WP_001178089.1 NZ_CP068040:c61121-60837 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT186521 NZ_CP089584:c1076655-1076404 [Xanthomonas translucens pv. undulosa]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGCGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAAGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References